Characteristics of Nipah virus and Hendra virus replication in different cell lines and their suitability for antiviral screening

Mohamad Aljofan, Simon Saubern, Adam Meyer, Glenn Marsh, Joanne Meers, Bruce Mungall

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28 Citations (Scopus)

Abstract

We have recently described the development and validation of a high throughput screening assay suitable for henipavirus antiviral identification. While we are confident this assay is robust and effective, we wished to investigate assay performance in a range of alternative cell lines to determine if assay sensitivity and specificity could be improved. We evaluated ten different cell lines for their susceptibility to Hendra and Nipah virus infection and their sensitivity of detection of the effects of the broad spectrum antiviral, ribavirin and nine novel antivirals identified using our initial screening approach. Cell lines were grouped into three categories with respect to viral replication. Virus replicated best in Vero and BSR cells, followed by Hep-2, HeLa, BHK-21 and M17 cells. The lowest levels of RNA replication and viral protein expression were observed in BAEC, MMEC, A549 and ECV304 cells. Eight cell lines appeared to be similarly effective at discriminating the antiviral effects of ribavirin (
Original languageEnglish
Pages (from-to)92 - 99
Number of pages8
JournalVirus Research
Volume142
Issue number1-2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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