Characterisation of the cell envelope of Treponema hyodysenteriae

Steven N. Chatfield, David S. Fernie, Julian Beesley, Charles Penn, Gordon Dougan

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4 Citations (Scopus)

Abstract

Cell envelope preparations of Treponema hyodysenteriae (strain CN 8368) were examined using biochemical and immunochemical methods. Several major polypeptides were detected with molecular weight between 24-kDa and 45-kDa. The majority of these polypeptides were recognised by serum from a pig vaccinated with an experimental whole-cell T. hyodysenteriae vaccine and hyperimmune anti-T. hyodysenteriae rabbit sera. Immune electron microscopy confirmed that the major antigens detected were associated with the cell envelope. Triton X-100, in the presence of EDTA, completely solubilised a polypeptide with an approximate molecular weight of 36-kDa. Antibodies to this polypeptide were not absorbed by whole T. innocens cells.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalFEMS Microbiology Letters
Volume55
Issue number3
DOIs
Publication statusPublished - 15 Oct 1988
Externally publishedYes

Keywords

  • Antigen
  • Cell envelope
  • Membrane protein
  • Treponema hydrosenteriae

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