Characterisation of the Activity and Stability of Single-chain Cathepsin L and of Proteolytically Active Cathepsin L/Cystatin Complexes

Clive Dennison, Robert Pike, Theresa Coetzer, Kirsten Kirk

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13 Citations (Scopus)

Abstract

The activity of single-chain cathepsin L was found to be markedly dependent on cysteine concentration, while a covalent, proteolytically active cathepsin L/ cystatin complex was less cysteine-dependent. Cysteine levels and ionic strength did not affect the stability of either enzyme form and both enzyme forms were found to be stable for significant periods of time at or near physiological pH.

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalBiological Chemistry Hoppe-Seyler
Volume373
Issue number2
DOIs
Publication statusPublished - 1 Jan 1992
Externally publishedYes

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