Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus

Ian T. Paulsen; Matthew T. Gillespie; Tim G. Littlejohn; Orawadee Hanvivatvong; Sally J. Rowland; Keith G.H. Dyke; Ronald A. Skurray

doi:10.1016/0378-1119(94)90136-8

Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus

Ian T. Paulsen, Matthew T. Gillespie, Tim G. Littlejohn, Orawadee Hanvivatvong, Sally J. Rowland, Keith G.H. Dyke, Ronald A. Skurray

Microbiology

Research output: Contribution to journal › Article › Research › peer-review

47 Citations (Scopus)

Abstract

The staphylococcal β-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pl9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on α-, β- and γ-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.

Original language	English
Pages (from-to)	109-114
Number of pages	6
Journal	Gene
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90136-8
Publication status	Published - 8 Apr 1994

Keywords

plasmids
Resolvases
transposon insertions
β-lactamase transposons

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10.1016/0378-1119(94)90136-8

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title = "Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus",

abstract = "The staphylococcal β-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pl9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on α-, β- and γ-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.",

keywords = "plasmids, Resolvases, transposon insertions, β-lactamase transposons",

author = "Paulsen, \{Ian T.\} and Gillespie, \{Matthew T.\} and Littlejohn, \{Tim G.\} and Orawadee Hanvivatvong and Rowland, \{Sally J.\} and Dyke, \{Keith G.H.\} and Skurray, \{Ronald A.\}",

year = "1994",

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doi = "10.1016/0378-1119(94)90136-8",

language = "English",

volume = "141",

pages = "109--114",

journal = "Gene",

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TY - JOUR

T1 - Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus

AU - Paulsen, Ian T.

AU - Gillespie, Matthew T.

AU - Littlejohn, Tim G.

AU - Hanvivatvong, Orawadee

AU - Rowland, Sally J.

AU - Dyke, Keith G.H.

AU - Skurray, Ronald A.

PY - 1994/4/8

Y1 - 1994/4/8

N2 - The staphylococcal β-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pl9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on α-, β- and γ-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.

AB - The staphylococcal β-lactamase (Bla) transposon Tn4002 has previously been reported to have a high level of insertional specificity for a 1.8-kb region on the plasmid pSK1. Nucleotide sequences of this region and of a related region on plasmid pl9789 were determined. Sequence analysis revealed that these two plasmids contain sin, a gene whose deduced product shows similarity to a family of DNA recombinases. Southern hybridisation analysis indicated that sin is located on α-, β- and γ-families of Bla plasmids and on pSK1 family plasmids. A region of dyad symmetry located upstream from sin on pSK1 and pI9789 was identified as the site of insertions of Tn552 and Tn4002 in separate isolates.

KW - plasmids

KW - Resolvases

KW - transposon insertions

KW - β-lactamase transposons

UR - https://www.scopus.com/pages/publications/0028204962

U2 - 10.1016/0378-1119(94)90136-8

DO - 10.1016/0378-1119(94)90136-8

M3 - Article

C2 - 8163161

AN - SCOPUS:0028204962

SN - 0378-1119

VL - 141

SP - 109

EP - 114

JO - Gene

JF - Gene

IS - 1

ER -

Characterisation of sin, a potential recombinase-encoding gene from Staphylococcus aureus

Abstract

Keywords

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