Characterisation of endothelin converting enzyme-1 shedding from endothelial cells

Don Monath Sanjaya Kuruppu, Shane Bernadine Reeve, Alexander Ian Smith

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

The aim of this study was to determine if endothelin converting enzyme-1 (ECE-1) like other members of this metalloprotease family undergoes ectodomain shedding. The release/shedding of catalytically active ECE-1 was measured by monitoring the fluorescence resulting from the cleavage of a specific quenched fluorescent substrate. Catalytically active ECE-1 was detected in the media of human umbilical vein endothelial cells, and was confirmed by mass spectrometry based assays. Specificity of cleavage was confirmed by using both narrow and broad specificity inhibitors. In conclusion we demonstrate and characterize for the first time, ECE-1 shedding from the surface of endothelial cells.
Original languageEnglish
Pages (from-to)4501 - 4506
Number of pages6
JournalFEBS Letters
Volume581
Issue number23
DOIs
Publication statusPublished - 2007

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