Characterisation of a Bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia

Mark Prescott; N. Matthew; H. Thorne; Andrew D. Milne; Alexander G. McLennan

doi:10.1016/0020-711X(92)90328-X

Characterisation of a Bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia

Mark Prescott, N. Matthew, H. Thorne, Andrew D. Milne, Alexander G. McLennan

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

1. 1. A P¹,P³-bis(5'-nucleosidy)triphosphate pyrophosphohydrolase (Np₃Nase) has been partially purified from Anemia embryos. 2. 2. The Np₃Nase has a native M_r of 115,000 and preferentially hydrolyses substrates of the form Np₃N. Relative rates of hydrolysis are Ap₃A (V_rel=1.0), Gp₃G (V_rel=0.71), Ap₄A (V_rel1 = 0.08), Ap₅A (v_rel = 0.09), Gp₄G (V_rel = 0.3) and Gp₅G (V_rel = 0.33). An NMP is always one of the products. 3. 3. The K_m values for Ap₃ and Gp₃G are 15 and 10μM respectively. 4. 4. Mg²⁺, Mn²⁺ and Ca²⁺ ions all stimulate the activity, while Zn²⁺, Co²⁺ and Ni²⁺ ions are inhibitory. 5. 5. The activity of the Np₃Nase remains constant during pre-emergence development of encysted embryos but decreases slightly after hatching.

Original language	English
Pages (from-to)	565-571
Number of pages	7
Journal	International Journal of Biochemistry
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/0020-711X(92)90328-X
Publication status	Published - Apr 1992
Externally published	Yes

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title = "Characterisation of a Bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia",

abstract = "1. 1. A P1,P3-bis(5'-nucleosidy)triphosphate pyrophosphohydrolase (Np3Nase) has been partially purified from Anemia embryos. 2. 2. The Np3Nase has a native Mr of 115,000 and preferentially hydrolyses substrates of the form Np3N. Relative rates of hydrolysis are Ap3A (Vrel=1.0), Gp3G (Vrel=0.71), Ap4A (Vrel1 = 0.08), Ap5A (vrel = 0.09), Gp4G (Vrel = 0.3) and Gp5G (Vrel = 0.33). An NMP is always one of the products. 3. 3. The Km values for Ap3 and Gp3G are 15 and 10μM respectively. 4. 4. Mg2+, Mn2+ and Ca2+ ions all stimulate the activity, while Zn2+, Co2+ and Ni2+ ions are inhibitory. 5. 5. The activity of the Np3Nase remains constant during pre-emergence development of encysted embryos but decreases slightly after hatching.",

author = "Mark Prescott and N. Matthew and H. Thorne and Milne, {Andrew D.} and McLennan, {Alexander G.}",

year = "1992",

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doi = "10.1016/0020-711X(92)90328-X",

language = "English",

volume = "24",

pages = "565--571",

journal = "International Journal of Biochemistry",

issn = "0020-711X",

publisher = "Elsevier",

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TY - JOUR

T1 - Characterisation of a Bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia

AU - Prescott, Mark

AU - Matthew, N.

AU - Thorne, H.

AU - Milne, Andrew D.

AU - McLennan, Alexander G.

PY - 1992/4

Y1 - 1992/4

N2 - 1. 1. A P1,P3-bis(5'-nucleosidy)triphosphate pyrophosphohydrolase (Np3Nase) has been partially purified from Anemia embryos. 2. 2. The Np3Nase has a native Mr of 115,000 and preferentially hydrolyses substrates of the form Np3N. Relative rates of hydrolysis are Ap3A (Vrel=1.0), Gp3G (Vrel=0.71), Ap4A (Vrel1 = 0.08), Ap5A (vrel = 0.09), Gp4G (Vrel = 0.3) and Gp5G (Vrel = 0.33). An NMP is always one of the products. 3. 3. The Km values for Ap3 and Gp3G are 15 and 10μM respectively. 4. 4. Mg2+, Mn2+ and Ca2+ ions all stimulate the activity, while Zn2+, Co2+ and Ni2+ ions are inhibitory. 5. 5. The activity of the Np3Nase remains constant during pre-emergence development of encysted embryos but decreases slightly after hatching.

AB - 1. 1. A P1,P3-bis(5'-nucleosidy)triphosphate pyrophosphohydrolase (Np3Nase) has been partially purified from Anemia embryos. 2. 2. The Np3Nase has a native Mr of 115,000 and preferentially hydrolyses substrates of the form Np3N. Relative rates of hydrolysis are Ap3A (Vrel=1.0), Gp3G (Vrel=0.71), Ap4A (Vrel1 = 0.08), Ap5A (vrel = 0.09), Gp4G (Vrel = 0.3) and Gp5G (Vrel = 0.33). An NMP is always one of the products. 3. 3. The Km values for Ap3 and Gp3G are 15 and 10μM respectively. 4. 4. Mg2+, Mn2+ and Ca2+ ions all stimulate the activity, while Zn2+, Co2+ and Ni2+ ions are inhibitory. 5. 5. The activity of the Np3Nase remains constant during pre-emergence development of encysted embryos but decreases slightly after hatching.

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U2 - 10.1016/0020-711X(92)90328-X

DO - 10.1016/0020-711X(92)90328-X

M3 - Article

C2 - 1325380

AN - SCOPUS:0026511334

SN - 0020-711X

VL - 24

SP - 565

EP - 571

JO - International Journal of Biochemistry

JF - International Journal of Biochemistry

IS - 4

ER -

Characterisation of a Bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia

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