Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell; Derek Pickard; John Wain; Keith James; L. T. Diem Nga; To Song Diep; Myron M. Levine; Peadar O'Gaora; Michael B. Prentice; Julian Parkhill; Nicholas Day; Jeremy Farrar; Gordon Dougan

doi:10.1016/S0147-619X(02)00013-6

Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell, Derek Pickard, John Wain, Keith James, L. T. Diem Nga, To Song Diep, Myron M. Levine, Peadar O'Gaora, Michael B. Prentice, Julian Parkhill, Nicholas Day, Jeremy Farrar, Gordon Dougan

Research output: Contribution to journal › Article › Research › peer-review

36 Citations (Scopus)

Abstract

pHCM2 is a 106kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

Original language	English
Pages (from-to)	159-171
Number of pages	13
Journal	Plasmid
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/S0147-619X(02)00013-6
Publication status	Published - 1 May 2002
Externally published	Yes

Keywords

Bacteriophage
Cryptic plasmids
Gene clusters
PCR
pFra plasmid
Salmonella typhi
Yersinia pestis

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10.1016/S0147-619X(02)00013-6

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@article{e67e2572515d43ad93a331c188a62c36,

title = "Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2",

abstract = "pHCM2 is a 106kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57\% of the coding sequences (CDSs) encoded on pHCM2 display over 97\% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.",

keywords = "Bacteriophage, Cryptic plasmids, Gene clusters, PCR, pFra plasmid, Salmonella typhi, Yersinia pestis",

author = "Claire Kidgell and Derek Pickard and John Wain and Keith James and \{Diem Nga\}, \{L. T.\} and Diep, \{To Song\} and Levine, \{Myron M.\} and Peadar O'Gaora and Prentice, \{Michael B.\} and Julian Parkhill and Nicholas Day and Jeremy Farrar and Gordon Dougan",

year = "2002",

month = may,

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doi = "10.1016/S0147-619X(02)00013-6",

language = "English",

volume = "47",

pages = "159--171",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press",

number = "3",

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TY - JOUR

T1 - Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

AU - Kidgell, Claire

AU - Pickard, Derek

AU - Wain, John

AU - James, Keith

AU - Diem Nga, L. T.

AU - Diep, To Song

AU - Levine, Myron M.

AU - O'Gaora, Peadar

AU - Prentice, Michael B.

AU - Parkhill, Julian

AU - Day, Nicholas

AU - Farrar, Jeremy

AU - Dougan, Gordon

PY - 2002/5/1

Y1 - 2002/5/1

N2 - pHCM2 is a 106kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

AB - pHCM2 is a 106kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

KW - Bacteriophage

KW - Cryptic plasmids

KW - Gene clusters

KW - PCR

KW - pFra plasmid

KW - Salmonella typhi

KW - Yersinia pestis

UR - https://www.scopus.com/pages/publications/0036557984

U2 - 10.1016/S0147-619X(02)00013-6

DO - 10.1016/S0147-619X(02)00013-6

M3 - Article

C2 - 12151231

AN - SCOPUS:0036557984

SN - 0147-619X

VL - 47

SP - 159

EP - 171

JO - Plasmid

JF - Plasmid

IS - 3

ER -

Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Abstract

Keywords

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