TY - JOUR
T1 - Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius)
AU - Dang, Mai
AU - Nowell, Cameron
AU - Nguyen, Tam
AU - Bach, Lis
AU - Sonne, Christian
AU - Nørregaard, Rasmus
AU - Stride, Megan
AU - Nowak, Barbara
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had spherical shape and limited variation in size (maximum diameter). We confirmed the close relationship between MMCs and blood vessels in spleen of shorthorn sculpins as 97% of investigated MMCs (60 whole MMCs over 510 μm thickness of the samples) were closely associated with splenic blood capillaries (mainly ellipsoids) at least once in a set of consecutive sections. In this paper, we describe variations in morphology, density, size, area, distribution, pigments and response to pathogens of MMC populations from different organs (spleen, kidney, liver, pancreas and gills). Additionally, we provide evidence suggesting the presence and dominance of pheomelanin in MMCs of shorthorn sculpins.
AB - Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had spherical shape and limited variation in size (maximum diameter). We confirmed the close relationship between MMCs and blood vessels in spleen of shorthorn sculpins as 97% of investigated MMCs (60 whole MMCs over 510 μm thickness of the samples) were closely associated with splenic blood capillaries (mainly ellipsoids) at least once in a set of consecutive sections. In this paper, we describe variations in morphology, density, size, area, distribution, pigments and response to pathogens of MMC populations from different organs (spleen, kidney, liver, pancreas and gills). Additionally, we provide evidence suggesting the presence and dominance of pheomelanin in MMCs of shorthorn sculpins.
KW - 3D structure
KW - Melanin
KW - Melanomacrophage centers
KW - Pheomelanin
KW - Shorthorn sculpin
UR - http://www.scopus.com/inward/record.url?scp=85061395922&partnerID=8YFLogxK
U2 - 10.1016/j.tice.2019.02.003
DO - 10.1016/j.tice.2019.02.003
M3 - Article
AN - SCOPUS:85061395922
SN - 0040-8166
VL - 57
SP - 34
EP - 41
JO - Tissue and Cell
JF - Tissue and Cell
ER -