Chapter 17 Determination of Yeast Mitochondrial KHE Activity, Osmotic Swelling and Mitophagy

Karin Nowikovsky, Rodney James Devenish, Elisabeth Froschauer, Rudolf J Schweyen

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The mitochondrial K(+)/H(+) exchanger (KHE) is a key regulator of mitochondrial K(+), the most abundant cellular cation, and thus for volume control of the organelle. Downregulation of the mitochondrial KHE results in osmotic swelling and autophagic degradation of the organelle. This chapter describes methods to shut-off expression of Mdm38p, an essential factor of the mitochondrial KHE, and to observe the cellular consequences thereof, in particular changes in KHE activity and morphogenetic changes of mitochondria by applying new techniques developed in our laboratories.
Original languageEnglish
Pages (from-to)305 - 317
Number of pages13
JournalMethods in Enzymology
Publication statusPublished - 2009

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