TY - JOUR
T1 - Chapter 17 Determination of Yeast Mitochondrial KHE Activity, Osmotic Swelling and Mitophagy
AU - Nowikovsky, Karin
AU - Devenish, Rodney James
AU - Froschauer, Elisabeth
AU - Schweyen, Rudolf J
PY - 2009
Y1 - 2009
N2 - The mitochondrial K(+)/H(+) exchanger (KHE) is a key regulator of mitochondrial K(+), the most abundant cellular cation, and thus for volume control of the organelle. Downregulation of the mitochondrial KHE results in osmotic swelling and autophagic degradation of the organelle. This chapter describes methods to shut-off expression of Mdm38p, an essential factor of the mitochondrial KHE, and to observe the cellular consequences thereof, in particular changes in KHE activity and morphogenetic changes of mitochondria by applying new techniques developed in our laboratories.
AB - The mitochondrial K(+)/H(+) exchanger (KHE) is a key regulator of mitochondrial K(+), the most abundant cellular cation, and thus for volume control of the organelle. Downregulation of the mitochondrial KHE results in osmotic swelling and autophagic degradation of the organelle. This chapter describes methods to shut-off expression of Mdm38p, an essential factor of the mitochondrial KHE, and to observe the cellular consequences thereof, in particular changes in KHE activity and morphogenetic changes of mitochondria by applying new techniques developed in our laboratories.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19426875
U2 - 10.1016/S0076-6879(09)05017-4
DO - 10.1016/S0076-6879(09)05017-4
M3 - Article
VL - 457
SP - 305
EP - 317
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
ER -