TY - JOUR
T1 - Changes in the extracellular matrix surrounding human chronic wounds revealed by 2-photon imaging
AU - Sutcliffe, Jessica E.S.
AU - Thrasivoulou, Christopher
AU - Serena, Thomas E.
AU - Madden, Leigh
AU - Richards, Toby
AU - Phillips, Anthony R.J.
AU - Becker, David L.
N1 - Publisher Copyright:
© 2017 Medicalhelplines.com Inc and John Wiley & Sons Ltd
PY - 2017/12
Y1 - 2017/12
N2 - Chronic wounds are a growing problem worldwide with no effective therapeutic treatments available. Our objective was to understand the composition of the dermal tissue surrounding venous leg ulcers and diabetic foot ulcers (DFU). We used novel 2-photon imaging techniques alongside classical histology to examine biopsies from the edges of two common types of chronic wound, venous leg ulcers and DFU. Compared to normal intact skin, we found that collagen levels are significantly reduced throughout the dermis of venous leg ulcer biopsies and DFU, with a reduction in both fibril thickness and abundance. Both wound types showed a significant reduction in elastin in the upper dermis, but in DFU, the loss was throughout the dermis. Loss of extracellular matrix correlated with high levels of CD68- and CD18-positive leukocytes. 2-photon imaging of the extracellular matrix in the intact tissue surrounding a chronic wound with a hand-held device may provide a useful clinical indicator on the healing progression or deterioration of these wounds.
AB - Chronic wounds are a growing problem worldwide with no effective therapeutic treatments available. Our objective was to understand the composition of the dermal tissue surrounding venous leg ulcers and diabetic foot ulcers (DFU). We used novel 2-photon imaging techniques alongside classical histology to examine biopsies from the edges of two common types of chronic wound, venous leg ulcers and DFU. Compared to normal intact skin, we found that collagen levels are significantly reduced throughout the dermis of venous leg ulcer biopsies and DFU, with a reduction in both fibril thickness and abundance. Both wound types showed a significant reduction in elastin in the upper dermis, but in DFU, the loss was throughout the dermis. Loss of extracellular matrix correlated with high levels of CD68- and CD18-positive leukocytes. 2-photon imaging of the extracellular matrix in the intact tissue surrounding a chronic wound with a hand-held device may provide a useful clinical indicator on the healing progression or deterioration of these wounds.
KW - Chronic wounds
KW - Extracellular matrix
KW - Second harmonic imaging
UR - http://www.scopus.com/inward/record.url?scp=85035145664&partnerID=8YFLogxK
U2 - 10.1111/iwj.12789
DO - 10.1111/iwj.12789
M3 - Article
C2 - 28730726
AN - SCOPUS:85035145664
SN - 1742-4801
VL - 14
SP - 1225
EP - 1236
JO - International Wound Journal
JF - International Wound Journal
IS - 6
ER -