Mitomycin C treatment of E. coli K 12 cells containing the nonconjugative plasmid CloDF13 resulted in inhibition of host chromosome protein synthesis and a high rate of synthesis of two CloDF13 specified proteins whose molecular weights correspond to cloacin and immunity protein. Five molecules of immunity protein were synthesized for each cloacin DF13 molecule. Mitomycin C treated cells containing a copy mutant of CloDF13 made three to four times as much of each protein as cells containing wild type CloDF13. CloDF13. CloDF13 plasmids that contained the transposon Tn1 were isolated. Two did not induce after mitomycin C treatment, failing both to inhibit host cell synthesis and to produce the two new proteins. In minicells, they showed reduced CloDF13 specified protein synthesis and produced three Tn1 specified proteins.
|Number of pages||6|
|Journal||Journal of Bacteriology|
|Publication status||Published - 1 Dec 1977|