Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model

Yutaka Fujii; Mikiyasu Shirai; James T Pearson; Yoshiaki Takewa; Eisuke Tatsumi

doi:10.1109/EMBC.2015.7318522

Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model

Yutaka Fujii, Mikiyasu Shirai, James T Pearson, Yoshiaki Takewa, Eisuke Tatsumi

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

4 Citations (Scopus)

Abstract

Cardiopulmonary bypass (CPB) is indispensable for cardiac surgery. Since the ethical and technical difficulties associated with clinical research limit the acquisition of new knowledge, it is desirable to have a miniature extracorporeal circulation (ECC) system for small animals. We aimed to establish a miniature ECC system and apply the system to the rat for investigating systemic inflammatory response changes during and after CPB. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m(2)), tubing line (polyvinyl chloride) and a roller pump. Priming volume of this system is only 7 ml. Rats were divided into the SHAM (received surgical preparation only without CPB) group and the CPB group. ECC pump flow was initiated and maintained at 70 ml/kg/min. We measured the serum cytokine levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-10, and biochemical markers (lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase) before, 30, and 60 min after the initiation of CPB, in addition, 30, 60, and 120 min after the CPB weaning. During CPB, blood pressure and hemoglobin were maintained around 80 mmHg and 10g/dl, the serum cytokine levels and biochemical markers were significantly elevated in the CPB group compared with the SHAM group. These data suggest that CPB promotes organ damage and a systemic inflammatory response. This rat ECC model is considered to be equivalent to the already established human CPB and useful for studying the mechanism of pathophysiological changes during and after CPB.

Original language	English
Title of host publication	2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015)
Editors	Nigel Lovell, Luca Mainardi
Place of Publication	Piscataway NJ USA
Publisher	IEEE, Institute of Electrical and Electronics Engineers
Pages	957-960
Number of pages	4
ISBN (Print)	9781424492695
DOIs	https://doi.org/10.1109/EMBC.2015.7318522
Publication status	Published - 2015
Event	International Conference of the IEEE Engineering in Medicine and Biology Society 2015: Biomedical Engineering: A bridge to improve the quality of health care and the quality of life - MiCo - Milano Congressi Center, Milan, Italy Duration: 25 Aug 2015 → 29 Aug 2015 Conference number: 37th https://embc.embs.org/2015/

Conference

Conference	International Conference of the IEEE Engineering in Medicine and Biology Society 2015
Abbreviated title	EMBC 2015
Country	Italy
City	Milan
Period	25/08/15 → 29/08/15
Other	37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS). Our Annual Conference (known as EMBC) is a premier international conference in biomedical engineering, and has been strategically held in different locations around the world, including Chicago, Osaka, San Diego, Boston, Buenos Aires, Minneapolis, Vancouver, Lyon, New York, and Shanghai over the last 10 years.
Internet address	https://embc.embs.org/2015/

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10.1109/EMBC.2015.7318522

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Cite this

Fujii, Y., Shirai, M., Pearson, J. T., Takewa, Y., & Tatsumi, E. (2015). Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model. In N. Lovell, & L. Mainardi (Eds.), 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015) (pp. 957-960). IEEE, Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/EMBC.2015.7318522

Fujii, Yutaka ; Shirai, Mikiyasu ; Pearson, James T ; Takewa, Yoshiaki ; Tatsumi, Eisuke. / Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015). editor / Nigel Lovell ; Luca Mainardi. Piscataway NJ USA : IEEE, Institute of Electrical and Electronics Engineers, 2015. pp. 957-960

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title = "Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model",

abstract = "Cardiopulmonary bypass (CPB) is indispensable for cardiac surgery. Since the ethical and technical difficulties associated with clinical research limit the acquisition of new knowledge, it is desirable to have a miniature extracorporeal circulation (ECC) system for small animals. We aimed to establish a miniature ECC system and apply the system to the rat for investigating systemic inflammatory response changes during and after CPB. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m(2)), tubing line (polyvinyl chloride) and a roller pump. Priming volume of this system is only 7 ml. Rats were divided into the SHAM (received surgical preparation only without CPB) group and the CPB group. ECC pump flow was initiated and maintained at 70 ml/kg/min. We measured the serum cytokine levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-10, and biochemical markers (lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase) before, 30, and 60 min after the initiation of CPB, in addition, 30, 60, and 120 min after the CPB weaning. During CPB, blood pressure and hemoglobin were maintained around 80 mmHg and 10g/dl, the serum cytokine levels and biochemical markers were significantly elevated in the CPB group compared with the SHAM group. These data suggest that CPB promotes organ damage and a systemic inflammatory response. This rat ECC model is considered to be equivalent to the already established human CPB and useful for studying the mechanism of pathophysiological changes during and after CPB.",

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Fujii, Y, Shirai, M, Pearson, JT, Takewa, Y & Tatsumi, E 2015, Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model. in N Lovell & L Mainardi (eds), 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015). IEEE, Institute of Electrical and Electronics Engineers, Piscataway NJ USA, pp. 957-960, International Conference of the IEEE Engineering in Medicine and Biology Society 2015, Milan, Italy, 25/08/15. https://doi.org/10.1109/EMBC.2015.7318522

Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model. / Fujii, Yutaka; Shirai, Mikiyasu; Pearson, James T; Takewa, Yoshiaki; Tatsumi, Eisuke.

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015). ed. / Nigel Lovell; Luca Mainardi. Piscataway NJ USA : IEEE, Institute of Electrical and Electronics Engineers, 2015. p. 957-960.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

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Fujii Y, Shirai M, Pearson JT, Takewa Y, Tatsumi E. Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model. In Lovell N, Mainardi L, editors, 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015). Piscataway NJ USA: IEEE, Institute of Electrical and Electronics Engineers. 2015. p. 957-960 https://doi.org/10.1109/EMBC.2015.7318522