Abstract
Cardiopulmonary bypass (CPB) is indispensable for cardiac surgery. Since the ethical and technical difficulties associated with clinical research limit the acquisition of new knowledge, it is desirable to have a miniature extracorporeal circulation (ECC) system for small animals. We aimed to establish a miniature ECC system and apply the system to the rat for investigating systemic inflammatory response changes during and after CPB. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m(2)), tubing line (polyvinyl chloride) and a roller pump. Priming volume of this system is only 7 ml. Rats were divided into the SHAM (received surgical preparation only without CPB) group and the CPB group. ECC pump flow was initiated and maintained at 70 ml/kg/min. We measured the serum cytokine levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-10, and biochemical markers (lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase) before, 30, and 60 min after the initiation of CPB, in addition, 30, 60, and 120 min after the CPB weaning. During CPB, blood pressure and hemoglobin were maintained around 80 mmHg and 10g/dl, the serum cytokine levels and biochemical markers were significantly elevated in the CPB group compared with the SHAM group. These data suggest that CPB promotes organ damage and a systemic inflammatory response. This rat ECC model is considered to be equivalent to the already established human CPB and useful for studying the mechanism of pathophysiological changes during and after CPB.
| Original language | English |
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| Title of host publication | 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015) |
| Editors | Nigel Lovell, Luca Mainardi |
| Place of Publication | Piscataway NJ USA |
| Publisher | IEEE, Institute of Electrical and Electronics Engineers |
| Pages | 957-960 |
| Number of pages | 4 |
| ISBN (Print) | 9781424492695 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | International Conference of the IEEE Engineering in Medicine and Biology Society 2015 - Milan, Italy Duration: 25 Aug 2015 → 29 Aug 2015 Conference number: 37th https://embc.embs.org/2015/ https://ieeexplore.ieee.org/xpl/conhome/7302811/proceeding (Proceedings) |
Conference
| Conference | International Conference of the IEEE Engineering in Medicine and Biology Society 2015 |
|---|---|
| Abbreviated title | EMBC 2015 |
| Country/Territory | Italy |
| City | Milan |
| Period | 25/08/15 → 29/08/15 |
| Other | 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS). Our Annual Conference (known as EMBC) is a premier international conference in biomedical engineering, and has been strategically held in different locations around the world, including Chicago, Osaka, San Diego, Boston, Buenos Aires, Minneapolis, Vancouver, Lyon, New York, and Shanghai over the last 10 years. |
| Internet address |