Changes in inflammatory response during and after cardiopulmonary bypass using a rat extracorporeal circulation model

Yutaka Fujii, Mikiyasu Shirai, James T Pearson, Yoshiaki Takewa, Eisuke Tatsumi

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Abstract

Cardiopulmonary bypass (CPB) is indispensable for cardiac surgery. Since the ethical and technical difficulties associated with clinical research limit the acquisition of new knowledge, it is desirable to have a miniature extracorporeal circulation (ECC) system for small animals. We aimed to establish a miniature ECC system and apply the system to the rat for investigating systemic inflammatory response changes during and after CPB. The ECC system consisted of a membranous oxygenator (polypropylene, 0.03 m(2)), tubing line (polyvinyl chloride) and a roller pump. Priming volume of this system is only 7 ml. Rats were divided into the SHAM (received surgical preparation only without CPB) group and the CPB group. ECC pump flow was initiated and maintained at 70 ml/kg/min. We measured the serum cytokine levels of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-10, and biochemical markers (lactate dehydrogenase, aspartate aminotransferase and alanine aminotransferase) before, 30, and 60 min after the initiation of CPB, in addition, 30, 60, and 120 min after the CPB weaning. During CPB, blood pressure and hemoglobin were maintained around 80 mmHg and 10g/dl, the serum cytokine levels and biochemical markers were significantly elevated in the CPB group compared with the SHAM group. These data suggest that CPB promotes organ damage and a systemic inflammatory response. This rat ECC model is considered to be equivalent to the already established human CPB and useful for studying the mechanism of pathophysiological changes during and after CPB.
Original languageEnglish
Title of host publication2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2015)
EditorsNigel Lovell, Luca Mainardi
Place of PublicationPiscataway NJ USA
PublisherIEEE, Institute of Electrical and Electronics Engineers
Pages957-960
Number of pages4
ISBN (Print)9781424492695
DOIs
Publication statusPublished - 2015
EventInternational Conference of the IEEE Engineering in Medicine and Biology Society 2015: Biomedical Engineering: A bridge to improve the quality of health care and the quality of life - MiCo - Milano Congressi Center, Milan, Italy
Duration: 25 Aug 201529 Aug 2015
Conference number: 37th
https://embc.embs.org/2015/

Conference

ConferenceInternational Conference of the IEEE Engineering in Medicine and Biology Society 2015
Abbreviated titleEMBC 2015
CountryItaly
CityMilan
Period25/08/1529/08/15
Other37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS). Our Annual Conference (known as EMBC) is a premier international conference in biomedical engineering, and has been strategically held in different locations around the world, including Chicago, Osaka, San Diego, Boston, Buenos Aires, Minneapolis, Vancouver, Lyon, New York, and Shanghai over the last 10 years.
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