Chain-specific antibodies for laminin-511

Laura Zamurs; Normand Pouliot; Nicole Kusuma; Edouard Nice; Anthony W Burgess

doi:10.3109/08977194.2013.859684

Chain-specific antibodies for laminin-511

Laura Zamurs, Normand Pouliot, Nicole Kusuma, Edouard Nice, Anthony W Burgess

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMalpha5 chain, 3G10 binds to the LMbeta1 chain and 3C12 binds to the LMgamma1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.

Original language	English
Pages (from-to)	209 - 219
Number of pages	11
Journal	Growth Factors
Volume	31
Issue number	6
DOIs	https://doi.org/10.3109/08977194.2013.859684
Publication status	Published - 2013

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10.3109/08977194.2013.859684

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title = "Chain-specific antibodies for laminin-511",

abstract = "Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMalpha5 chain, 3G10 binds to the LMbeta1 chain and 3C12 binds to the LMgamma1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.",

author = "Laura Zamurs and Normand Pouliot and Nicole Kusuma and Edouard Nice and Burgess, {Anthony W}",

year = "2013",

doi = "10.3109/08977194.2013.859684",

language = "English",

volume = "31",

pages = "209 -- 219",

journal = "Growth Factors",

issn = "0897-7194",

publisher = "Taylor & Francis",

number = "6",

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TY - JOUR

T1 - Chain-specific antibodies for laminin-511

AU - Zamurs, Laura

AU - Pouliot, Normand

AU - Kusuma, Nicole

AU - Nice, Edouard

AU - Burgess, Anthony W

PY - 2013

Y1 - 2013

N2 - Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMalpha5 chain, 3G10 binds to the LMbeta1 chain and 3C12 binds to the LMgamma1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.

AB - Mouse monoclonal antibodies (mAbs) that bind to specific chains of laminin-511 (LM-511) have been developed. Antibody 2F12 binds to the LMalpha5 chain, 3G10 binds to the LMbeta1 chain and 3C12 binds to the LMgamma1 chain. These antibodies can be used to purify LM-511, to detect LM-511 in cell extracts or to detect the location of LM-511 in tissue by immunohistochemistry. In combination, the antibodies recognize all three chains of LM-511 and combinations of the antibodies can be used to quantitate levels of LM-511 in physiological fluids. One of the antibodies (3G10) is a potent inhibitor of the activity of LM-511 in cell adhesion, spreading and proliferation assays.

UR - http://www.ncbi.nlm.nih.gov/pubmed/24274106

U2 - 10.3109/08977194.2013.859684

DO - 10.3109/08977194.2013.859684

M3 - Article

VL - 31

SP - 209

EP - 219

JO - Growth Factors

JF - Growth Factors

SN - 0897-7194

IS - 6

ER -

Chain-specific antibodies for laminin-511

Abstract

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