Central infusion of leptin does not increase AMPK signaling in skeletal muscle of sheep

Rhianna C Laker; Belinda A Henry; Glenn D Wadley; Iain J Clarke; Benedict J Canny; Glenn K McConell

doi:10.1152/ajpregu.00079.2010

Central infusion of leptin does not increase AMPK signaling in skeletal muscle of sheep

Rhianna C Laker, Belinda A Henry, Glenn D Wadley, Iain J Clarke, Benedict J Canny, Glenn K McConell

Physiology

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

In sheep, central leptin infusion reduces food intake and increases energy expenditure in adipose tissue and skeletal muscle. The mechanism(s) for these peripheral effects of central leptin in sheep are not known but, based on rodent studies, may involve AMP-activated protein kinase (AMPK). In mice, central leptin acutely increases both skeletal muscle AMPK activation and glucose uptake. Thus, to investigate if these effects exist in higher order mammals, ovariectomized Corriedale ewes (N=4 per group) received a continuous lateral ventricular infusion (60mul/h) of either leptin (50mug/h) or artificial cerebrospinal fluid (aCSF, CON) for 8 days. Tritiated glucose (3-(3)H-glucose) was infused intravenously for calculation of whole body glucose turnover during both acute (6 h) and chronic (7-8 days) leptin/aCSF infusion. Muscle biopsies were also obtained. Leptin infusion reduced (P

Original language	English
Pages (from-to)	R511 - R518
Number of pages	8
Journal	American Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume	300
Issue number	2
DOIs	https://doi.org/10.1152/ajpregu.00079.2010
Publication status	Published - 2011

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Cite this

@article{6f3ee00bd1a849128c81749c90104cc0,

title = "Central infusion of leptin does not increase AMPK signaling in skeletal muscle of sheep",

abstract = "In sheep, central leptin infusion reduces food intake and increases energy expenditure in adipose tissue and skeletal muscle. The mechanism(s) for these peripheral effects of central leptin in sheep are not known but, based on rodent studies, may involve AMP-activated protein kinase (AMPK). In mice, central leptin acutely increases both skeletal muscle AMPK activation and glucose uptake. Thus, to investigate if these effects exist in higher order mammals, ovariectomized Corriedale ewes (N=4 per group) received a continuous lateral ventricular infusion (60mul/h) of either leptin (50mug/h) or artificial cerebrospinal fluid (aCSF, CON) for 8 days. Tritiated glucose (3-(3)H-glucose) was infused intravenously for calculation of whole body glucose turnover during both acute (6 h) and chronic (7-8 days) leptin/aCSF infusion. Muscle biopsies were also obtained. Leptin infusion reduced (P",

author = "Laker, {Rhianna C} and Henry, {Belinda A} and Wadley, {Glenn D} and Clarke, {Iain J} and Canny, {Benedict J} and McConell, {Glenn K}",

year = "2011",

doi = "10.1152/ajpregu.00079.2010",

language = "English",

volume = "300",

pages = "R511 -- R518",

journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",

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publisher = "American Physiological Society",

number = "2",

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Central infusion of leptin does not increase AMPK signaling in skeletal muscle of sheep. / Laker, Rhianna C; Henry, Belinda A; Wadley, Glenn D; Clarke, Iain J ; Canny, Benedict J; McConell, Glenn K.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 300, No. 2, 2011, p. R511 - R518.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Central infusion of leptin does not increase AMPK signaling in skeletal muscle of sheep

AU - Laker, Rhianna C

AU - Henry, Belinda A

AU - Wadley, Glenn D

AU - Clarke, Iain J

AU - Canny, Benedict J

AU - McConell, Glenn K

PY - 2011

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AB - In sheep, central leptin infusion reduces food intake and increases energy expenditure in adipose tissue and skeletal muscle. The mechanism(s) for these peripheral effects of central leptin in sheep are not known but, based on rodent studies, may involve AMP-activated protein kinase (AMPK). In mice, central leptin acutely increases both skeletal muscle AMPK activation and glucose uptake. Thus, to investigate if these effects exist in higher order mammals, ovariectomized Corriedale ewes (N=4 per group) received a continuous lateral ventricular infusion (60mul/h) of either leptin (50mug/h) or artificial cerebrospinal fluid (aCSF, CON) for 8 days. Tritiated glucose (3-(3)H-glucose) was infused intravenously for calculation of whole body glucose turnover during both acute (6 h) and chronic (7-8 days) leptin/aCSF infusion. Muscle biopsies were also obtained. Leptin infusion reduced (P

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