Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems

Vinod Balasubramaniam; Sharifah Syed Hassan; Abdul Rahman Omar; Maizan Mohamed; Suriani M Noor; Ramlan Mohamed; Iekhsan Othman

doi:10.1186/1745-422X-8-196

Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems

Vinod Balasubramaniam, Sharifah Syed Hassan, Abdul Rahman Omar, Maizan Mohamed, Suriani M Noor, Ramlan Mohamed, Iekhsan Othman

Malaysia Jeffrey Cheah Sch of Med & HS

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing ) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan- based real time quantitative PCR assay.

Original language	English
Pages (from-to)	1 - 13
Number of pages	13
Journal	Virology Journal
Volume	8
DOIs	https://doi.org/10.1186/1745-422X-8-196
Publication status	Published - 2011

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10.1186/1745-422X-8-196

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title = "Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems",

abstract = "Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing ) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan- based real time quantitative PCR assay.",

author = "Vinod Balasubramaniam and Hassan, {Sharifah Syed} and Omar, {Abdul Rahman} and Maizan Mohamed and Noor, {Suriani M} and Ramlan Mohamed and Iekhsan Othman",

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T1 - Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems

AU - Balasubramaniam, Vinod

AU - Hassan, Sharifah Syed

AU - Omar, Abdul Rahman

AU - Mohamed, Maizan

AU - Noor, Suriani M

AU - Mohamed, Ramlan

AU - Othman, Iekhsan

PY - 2011

Y1 - 2011

N2 - Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing ) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan- based real time quantitative PCR assay.

AB - Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing ) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan- based real time quantitative PCR assay.

UR - http://www.virologyj.com/content/8/1/196

U2 - 10.1186/1745-422X-8-196

DO - 10.1186/1745-422X-8-196

M3 - Article

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VL - 8

SP - 1

EP - 13

JO - Virology Journal

JF - Virology Journal

ER -

Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems

Abstract

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