Cellular transcripts regulated during infections with highly pathogenic H5N1 Avian Influenza virus in 3 host systems

Vinod Balasubramaniam, Sharifah Syed Hassan, Abdul Rahman Omar, Maizan Mohamed, Suriani M Noor, Ramlan Mohamed, Iekhsan Othman

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    Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu) and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing ) using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs). Seven of the genes have been selected for validation using a TaqMan- based real time quantitative PCR assay.
    Original languageEnglish
    Pages (from-to)1 - 13
    Number of pages13
    JournalVirology Journal
    Publication statusPublished - 2011

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