TY - JOUR
T1 - Cellular localization of the chemokine receptor CCR5
T2 - Correlation to cellular targets of HIV-1 infection
AU - Rottman, James B.
AU - Ganley, Kenneth P.
AU - Williams, Kenneth
AU - Wu, Lijun
AU - Mackay, Charles R.
AU - Ringler, Douglas J.
PY - 1997/11/1
Y1 - 1997/11/1
N2 - The chemokine receptor CCR5 has recently been described as a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1. In this study, using a panel of monoclonal antibodies specific for human CCR5, we show by immunohistochemistry and flow cytometry that CCR5 is expressed by bone-marrow-derived cells known to be targets for HIV-1 infection, including a subpopulation of lymphocytes and monocyte/macrophages in blood, primary and secondary lymphoid organs, and noninflamed tissues. In the central nervous system, CCR5 is expressed on neurons, astrocytes, and microglia. In other tissues, CCR5 is expressed on epithelium, endothelium, vascular smooth muscle, and fibroblasts. Chronically inflamed tissues contain an increased number of CCR5+ mononuclear cells, and the number of immunoreactive cells is directly associated with a histopathological correlate of inflammatory severity. Collectively, these results suggest that CCR5+ cells are recruited to inflammatory sites and, as such, may facilitate transmission of macrophagetropic strains of HIV-1.
AB - The chemokine receptor CCR5 has recently been described as a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1. In this study, using a panel of monoclonal antibodies specific for human CCR5, we show by immunohistochemistry and flow cytometry that CCR5 is expressed by bone-marrow-derived cells known to be targets for HIV-1 infection, including a subpopulation of lymphocytes and monocyte/macrophages in blood, primary and secondary lymphoid organs, and noninflamed tissues. In the central nervous system, CCR5 is expressed on neurons, astrocytes, and microglia. In other tissues, CCR5 is expressed on epithelium, endothelium, vascular smooth muscle, and fibroblasts. Chronically inflamed tissues contain an increased number of CCR5+ mononuclear cells, and the number of immunoreactive cells is directly associated with a histopathological correlate of inflammatory severity. Collectively, these results suggest that CCR5+ cells are recruited to inflammatory sites and, as such, may facilitate transmission of macrophagetropic strains of HIV-1.
UR - http://www.scopus.com/inward/record.url?scp=0030698047&partnerID=8YFLogxK
M3 - Article
C2 - 9358760
AN - SCOPUS:0030698047
SN - 0002-9440
VL - 151
SP - 1341
EP - 1351
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 5
ER -
