Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

Simon C. Baker; Geraldine Rohman; Jennifer Hinley; Jens Stahlschmidt; Neil R. Cameron; Jennifer Southgate

doi:10.1002/mabi.201000415

Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

Simon C. Baker
, Geraldine Rohman
, Jennifer Hinley
, Jens Stahlschmidt
, Neil R. Cameron
, Jennifer Southgate

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68⁺ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

Original language	English
Pages (from-to)	618-627
Number of pages	10
Journal	Macromolecular Bioscience
Volume	11
Issue number	5
DOIs	https://doi.org/10.1002/mabi.201000415
Publication status	Published - 12 May 2011
Externally published	Yes

Keywords

Biomaterials
CD163
CD68
Macrophages
Polyesters

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10.1002/mabi.201000415

Cite this

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title = "Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold",

abstract = "Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.",

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AU - Rohman, Geraldine

AU - Hinley, Jennifer

AU - Stahlschmidt, Jens

AU - Cameron, Neil R.

AU - Southgate, Jennifer

PY - 2011/5/12

Y1 - 2011/5/12

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AB - Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

KW - Biomaterials

KW - CD163

KW - CD68

KW - Macrophages

KW - Polyesters

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M3 - Article

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SN - 1616-5187

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JO - Macromolecular Bioscience

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IS - 5

ER -

Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

Abstract

Keywords

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