Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

Simon C. Baker, Geraldine Rohman, Jennifer Hinley, Jens Stahlschmidt, Neil R. Cameron, Jennifer Southgate

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

Original languageEnglish
Pages (from-to)618-627
Number of pages10
JournalMacromolecular Bioscience
Volume11
Issue number5
DOIs
Publication statusPublished - 12 May 2011
Externally publishedYes

Keywords

  • Biomaterials
  • CD163
  • CD68
  • Macrophages
  • Polyesters

Cite this

Baker, Simon C. ; Rohman, Geraldine ; Hinley, Jennifer ; Stahlschmidt, Jens ; Cameron, Neil R. ; Southgate, Jennifer. / Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold. In: Macromolecular Bioscience. 2011 ; Vol. 11, No. 5. pp. 618-627.
@article{bc59837704a04d0e9643afb1db7fd0a0,
title = "Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold",
abstract = "Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.",
keywords = "Biomaterials, CD163, CD68, Macrophages, Polyesters",
author = "Baker, {Simon C.} and Geraldine Rohman and Jennifer Hinley and Jens Stahlschmidt and Cameron, {Neil R.} and Jennifer Southgate",
year = "2011",
month = "5",
day = "12",
doi = "10.1002/mabi.201000415",
language = "English",
volume = "11",
pages = "618--627",
journal = "Macromolecular Bioscience",
issn = "1616-5187",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "5",

}

Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold. / Baker, Simon C.; Rohman, Geraldine; Hinley, Jennifer; Stahlschmidt, Jens; Cameron, Neil R.; Southgate, Jennifer.

In: Macromolecular Bioscience, Vol. 11, No. 5, 12.05.2011, p. 618-627.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

AU - Baker, Simon C.

AU - Rohman, Geraldine

AU - Hinley, Jennifer

AU - Stahlschmidt, Jens

AU - Cameron, Neil R.

AU - Southgate, Jennifer

PY - 2011/5/12

Y1 - 2011/5/12

N2 - Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

AB - Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

KW - Biomaterials

KW - CD163

KW - CD68

KW - Macrophages

KW - Polyesters

UR - http://www.scopus.com/inward/record.url?scp=79955725549&partnerID=8YFLogxK

U2 - 10.1002/mabi.201000415

DO - 10.1002/mabi.201000415

M3 - Article

VL - 11

SP - 618

EP - 627

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

SN - 1616-5187

IS - 5

ER -