Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold

Simon C. Baker, Geraldine Rohman, Jennifer Hinley, Jens Stahlschmidt, Neil R. Cameron, Jennifer Southgate

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200μm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.

Original languageEnglish
Pages (from-to)618-627
Number of pages10
JournalMacromolecular Bioscience
Volume11
Issue number5
DOIs
Publication statusPublished - 12 May 2011
Externally publishedYes

Keywords

  • Biomaterials
  • CD163
  • CD68
  • Macrophages
  • Polyesters

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