Cellular and molecular characterisation of the ovine rectal mucosal environment

Bradley J Sedgmen, Shari Lofthouse, Jean-Pierre Yves Scheerlinck, Elza Nicole Theresia Meeusen

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Immunohistological characterisation of ovine rectal tissue has revealed the presence of lymphoid follicles, predominantly in the submucosa, that closely resemble those found in intestinal Peyer s patches (PPs). Distinct T (CD4+, CD8+, gammadelta-TCR+) and B (CD21+, CD45R+) lymphocyte staining patterns were observed within and around follicles of the rectal mucosa. In addition, IgA+ and IgE+ cells were also found at this tissue site, with both phenotypes commonly residing in the lamina propria. RT-PCR examination of the cytokines expressed in the rectal mucosal tissue revealed consistently high levels of TGFbeta and IL-8 mRNA, low levels of IL-2 mRNA and no detectable IL-4 mRNA. The presence of lymphoid follicles, IgA+ plasma cells and IgA-inducing cytokines in rectal tissue of sheep indicate that this may be a suitable route for delivering mucosal vaccines.
Original languageEnglish
Pages (from-to)215 - 220
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume86
Issue number3-4
Publication statusPublished - 2002

Cite this