Cell Type of Origin Dictates the Route to Pluripotency

Christian M. Nefzger, Fernando J. Rossello, Joseph Chen, Xiaodong Liu, Anja S. Knaupp, Jaber Firas, Jacob M. Paynter, Jahnvi Pflueger, Sam Buckberry, Sue Mei Lim, Brenda Williams, Sara Alaei, Keshav Faye-Chauhan, Enrico Petretto, Susan K. Nilsson, Ryan Lister, Mirana Ramialison, David R. Powell, Owen J.L. Rackham, Jose M. Polo

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources. Nefzger et al. find that the molecular reprogramming trajectories of fibroblasts, neutrophils, and keratinocytes have a cell-type-specific component that only fully converges in induced pluripotent stem cells. The authors also identify universal changes shared by all three cell types, including two transcriptional waves and a conserved transcriptional program involving Egr1 downregulation.

LanguageEnglish
Pages2649-2660
Number of pages12
JournalCell Reports
Volume21
Issue number10
DOIs
Publication statusPublished - 5 Dec 2017

Keywords

  • Egr1
  • fibroblasts
  • induced pluripotent stem cells
  • keratinocytes
  • neutrophils
  • reprogramming
  • transcriptional dynamics

Cite this

Nefzger, Christian M. ; Rossello, Fernando J. ; Chen, Joseph ; Liu, Xiaodong ; Knaupp, Anja S. ; Firas, Jaber ; Paynter, Jacob M. ; Pflueger, Jahnvi ; Buckberry, Sam ; Lim, Sue Mei ; Williams, Brenda ; Alaei, Sara ; Faye-Chauhan, Keshav ; Petretto, Enrico ; Nilsson, Susan K. ; Lister, Ryan ; Ramialison, Mirana ; Powell, David R. ; Rackham, Owen J.L. ; Polo, Jose M. / Cell Type of Origin Dictates the Route to Pluripotency. In: Cell Reports. 2017 ; Vol. 21, No. 10. pp. 2649-2660.
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abstract = "Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources. Nefzger et al. find that the molecular reprogramming trajectories of fibroblasts, neutrophils, and keratinocytes have a cell-type-specific component that only fully converges in induced pluripotent stem cells. The authors also identify universal changes shared by all three cell types, including two transcriptional waves and a conserved transcriptional program involving Egr1 downregulation.",
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Nefzger, CM, Rossello, FJ, Chen, J, Liu, X, Knaupp, AS, Firas, J, Paynter, JM, Pflueger, J, Buckberry, S, Lim, SM, Williams, B, Alaei, S, Faye-Chauhan, K, Petretto, E, Nilsson, SK, Lister, R, Ramialison, M, Powell, DR, Rackham, OJL & Polo, JM 2017, 'Cell Type of Origin Dictates the Route to Pluripotency' Cell Reports, vol. 21, no. 10, pp. 2649-2660. https://doi.org/10.1016/j.celrep.2017.11.029

Cell Type of Origin Dictates the Route to Pluripotency. / Nefzger, Christian M.; Rossello, Fernando J.; Chen, Joseph; Liu, Xiaodong; Knaupp, Anja S.; Firas, Jaber; Paynter, Jacob M.; Pflueger, Jahnvi; Buckberry, Sam; Lim, Sue Mei; Williams, Brenda; Alaei, Sara; Faye-Chauhan, Keshav; Petretto, Enrico; Nilsson, Susan K.; Lister, Ryan; Ramialison, Mirana; Powell, David R.; Rackham, Owen J.L.; Polo, Jose M.

In: Cell Reports, Vol. 21, No. 10, 05.12.2017, p. 2649-2660.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Cell Type of Origin Dictates the Route to Pluripotency

AU - Nefzger, Christian M.

AU - Rossello, Fernando J.

AU - Chen, Joseph

AU - Liu, Xiaodong

AU - Knaupp, Anja S.

AU - Firas, Jaber

AU - Paynter, Jacob M.

AU - Pflueger, Jahnvi

AU - Buckberry, Sam

AU - Lim, Sue Mei

AU - Williams, Brenda

AU - Alaei, Sara

AU - Faye-Chauhan, Keshav

AU - Petretto, Enrico

AU - Nilsson, Susan K.

AU - Lister, Ryan

AU - Ramialison, Mirana

AU - Powell, David R.

AU - Rackham, Owen J.L.

AU - Polo, Jose M.

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N2 - Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources. Nefzger et al. find that the molecular reprogramming trajectories of fibroblasts, neutrophils, and keratinocytes have a cell-type-specific component that only fully converges in induced pluripotent stem cells. The authors also identify universal changes shared by all three cell types, including two transcriptional waves and a conserved transcriptional program involving Egr1 downregulation.

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KW - keratinocytes

KW - neutrophils

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KW - transcriptional dynamics

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