Cell type boundaries organize plant development

Monica Pia Caggiano, Xiulian Yu, Neha Bhatia, Andre Larsson, Hasthi Ram, Carolyn K. Ohno, Pia Sappl, Elliot M. Meyerowitz, Henrik Jönsson, Marcus G. Heisler

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.

Original languageEnglish
Article numbere27421
Number of pages32
JournaleLife
Volume6
DOIs
Publication statusPublished - 12 Sep 2017
Externally publishedYes

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