Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation

Ya-li Li; Guang-zhi Wu; Li Zeng; Gavin S Dawe; Li Sun; Gabriele Loers; Thomas Tilling; Shu-sen Cui; Melitta Schachner; Zhi-Cheng Xiao

doi:10.1016/j.febslet.2009.01.013

Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation

Ya-li Li, Guang-zhi Wu, Li Zeng, Gavin S Dawe, Li Sun, Gabriele Loers, Thomas Tilling, Shu-sen Cui, Melitta Schachner, Zhi-Cheng Xiao

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.

Original language	English
Pages (from-to)	703 - 710
Number of pages	8
Journal	FEBS Letters
Volume	583
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2009.01.013
Publication status	Published - 2009
Externally published	Yes

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10.1016/j.febslet.2009.01.013

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Li, Y., Wu, G., Zeng, L., Dawe, G. S., Sun, L., Loers, G., Tilling, T., Cui, S., Schachner, M., & Xiao, Z.-C. (2009). Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation. FEBS Letters, 583(4), 703 - 710. https://doi.org/10.1016/j.febslet.2009.01.013

@article{8639a584f8174d058bea1b02360b71f6,

title = "Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation",

abstract = "Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.",

author = "Ya-li Li and Guang-zhi Wu and Li Zeng and Dawe, {Gavin S} and Li Sun and Gabriele Loers and Thomas Tilling and Shu-sen Cui and Melitta Schachner and Zhi-Cheng Xiao",

year = "2009",

doi = "10.1016/j.febslet.2009.01.013",

language = "English",

volume = "583",

pages = "703 -- 710",

journal = "FEBS Letters",

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publisher = "John Wiley & Sons",

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}

Li, Y, Wu, G, Zeng, L, Dawe, GS, Sun, L, Loers, G, Tilling, T, Cui, S, Schachner, M & Xiao, Z-C 2009, 'Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation', FEBS Letters, vol. 583, no. 4, pp. 703 - 710. https://doi.org/10.1016/j.febslet.2009.01.013

TY - JOUR

T1 - Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation

AU - Li, Ya-li

AU - Wu, Guang-zhi

AU - Zeng, Li

AU - Dawe, Gavin S

AU - Sun, Li

AU - Loers, Gabriele

AU - Tilling, Thomas

AU - Cui, Shu-sen

AU - Schachner, Melitta

AU - Xiao, Zhi-Cheng

PY - 2009

Y1 - 2009

N2 - Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.

AB - Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19166842

U2 - 10.1016/j.febslet.2009.01.013

DO - 10.1016/j.febslet.2009.01.013

M3 - Article

SN - 0014-5793

VL - 583

SP - 703

EP - 710

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -

Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLC? and cooperate to modulate embryonic stem cell survival and proliferation

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