Cell size, photosynthesis and the package effect

an artificial selection approach

Martino E. Malerba, Maria M. Palacios, Yussi M. Palacios Delgado, John Beardall, Dustin J. Marshall

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cell size correlates with most traits among phytoplankton species. Theory predicts that larger cells should show poorer photosynthetic performance, perhaps due to reduced intracellular self-shading (i.e. package effect). Yet current theory relies heavily on interspecific correlational approaches and causal relationships between size and photosynthetic machinery have remained untested. 

As a more direct test, we applied 250 generations of artificial selection (c. 20 months) to evolve the green microalga Dunaliella teriolecta (Chlorophyta) toward different mean cell sizes, while monitoring all major photosynthetic parameters. 

Evolving larger sizes (> 1500% difference in volume) resulted in reduced oxygen production per chlorophyll molecule – as predicted by the package effect. However, large-evolved cells showed substantially higher rates of oxygen production – a finding unanticipated by current theory. In addition, volume-specific photosynthetic pigments increased with size (Chla+b), while photo-protectant pigments decreased (β-carotene). Finally, larger cells displayed higher growth performances and Fv/Fm, steeper slopes of rapid light curves (α) and smaller light-harvesting antennae (σPSII) with higher connectivity (ρ). 

Overall, evolving a common ancestor into different sizes showed that the photosynthetic characteristics of a species coevolves with cell volume. Moreover, our experiment revealed a trade-off between chlorophyll-specific (decreasing with size) and volume-specific (increasing with size) oxygen production in a cell.

Original languageEnglish
Pages (from-to)449-461
Number of pages13
JournalNew Phytologist
Volume219
Issue number1
DOIs
Publication statusPublished - 1 Jul 2018

Keywords

  • allometric size-scaling
  • artificial selection
  • evolutionary size-shift
  • experimental evolution
  • geometric biology
  • metabolism
  • oxygen evolution
  • primary production

Cite this

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abstract = "Cell size correlates with most traits among phytoplankton species. Theory predicts that larger cells should show poorer photosynthetic performance, perhaps due to reduced intracellular self-shading (i.e. package effect). Yet current theory relies heavily on interspecific correlational approaches and causal relationships between size and photosynthetic machinery have remained untested. As a more direct test, we applied 250 generations of artificial selection (c. 20 months) to evolve the green microalga Dunaliella teriolecta (Chlorophyta) toward different mean cell sizes, while monitoring all major photosynthetic parameters. Evolving larger sizes (> 1500{\%} difference in volume) resulted in reduced oxygen production per chlorophyll molecule – as predicted by the package effect. However, large-evolved cells showed substantially higher rates of oxygen production – a finding unanticipated by current theory. In addition, volume-specific photosynthetic pigments increased with size (Chla+b), while photo-protectant pigments decreased (β-carotene). Finally, larger cells displayed higher growth performances and Fv/Fm, steeper slopes of rapid light curves (α) and smaller light-harvesting antennae (σPSII) with higher connectivity (ρ). Overall, evolving a common ancestor into different sizes showed that the photosynthetic characteristics of a species coevolves with cell volume. Moreover, our experiment revealed a trade-off between chlorophyll-specific (decreasing with size) and volume-specific (increasing with size) oxygen production in a cell.",
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Cell size, photosynthesis and the package effect : an artificial selection approach. / Malerba, Martino E.; Palacios, Maria M.; Palacios Delgado, Yussi M.; Beardall, John; Marshall, Dustin J.

In: New Phytologist, Vol. 219, No. 1, 01.07.2018, p. 449-461.

Research output: Contribution to journalArticleResearchpeer-review

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