TY - JOUR
T1 - Cell recognition molecule L1 promotes embryonic stem cell differentiation through the regulation of cell surface glycosylation
AU - Li, Ying
AU - Huang, Xiaohua
AU - An, Yue
AU - Ren, Feng
AU - Yang, Zara Zhuyun
AU - Zhu, Hongmei
AU - Zhou, Lei
AU - He, Xiaowen
AU - Schachner, Melitta
AU - Xiao, Zhi-Cheng
AU - Ma, Keli
AU - Li, Yali
PY - 2013
Y1 - 2013
N2 - Cell recognition molecule L1 (CD171) plays an important role in neuronal survival, migration, differentiation, neurite outgrowth, myelination, synaptic plasticity and regeneration after injury. Our previous study has demonstrated that overexpressing L1 enhances cell survival and proliferation of mouse embryonic stem cells (ESCs) through promoting the expression of FUT9 and ST3Gal4, which upregulates cell surface sialylation and fucosylation. In the present study, we examined whether sialylation and fucosylation are involved in ESC differentiation through L1 signaling. RNA interference analysis showed that L1 enhanced differentiation of ESCs into neurons through the upregulation of FUT9 and ST3Gal4. Furthermore, blocking the phospholipase Cgamma (PLCgamma) signaling pathway with either a specific PLCgamma inhibitor or knockdown PLCgamma reduced the expression levels of both FUT9 and ST3Gal4 mRNAs and inhibited L1-mediated neuronal differentiation. These results demonstrate that L1 promotes neuronal differentiation from ESCs through the L1-mediated enhancement of FUT9 and ST3Gal4 expression.
AB - Cell recognition molecule L1 (CD171) plays an important role in neuronal survival, migration, differentiation, neurite outgrowth, myelination, synaptic plasticity and regeneration after injury. Our previous study has demonstrated that overexpressing L1 enhances cell survival and proliferation of mouse embryonic stem cells (ESCs) through promoting the expression of FUT9 and ST3Gal4, which upregulates cell surface sialylation and fucosylation. In the present study, we examined whether sialylation and fucosylation are involved in ESC differentiation through L1 signaling. RNA interference analysis showed that L1 enhanced differentiation of ESCs into neurons through the upregulation of FUT9 and ST3Gal4. Furthermore, blocking the phospholipase Cgamma (PLCgamma) signaling pathway with either a specific PLCgamma inhibitor or knockdown PLCgamma reduced the expression levels of both FUT9 and ST3Gal4 mRNAs and inhibited L1-mediated neuronal differentiation. These results demonstrate that L1 promotes neuronal differentiation from ESCs through the L1-mediated enhancement of FUT9 and ST3Gal4 expression.
UR - http://www.ncbi.nlm.nih.gov/pubmed/24070611
U2 - 10.1016/j.bbrc.2013.09.082
DO - 10.1016/j.bbrc.2013.09.082
M3 - Article
SN - 0006-291X
VL - 440
SP - 405
EP - 412
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -