Cell microencapsulation within gelatin-PEG microgels using a simple pipet tip-based device

Thuy P.T. Nguyen; Fanyi Li; Brendan Hung; Vinh Xuan Truong; Helmut Thissen; John S. Forsythe; Jessica E. Frith

doi:10.1021/acsbiomaterials.3c00676

Cell microencapsulation within gelatin-PEG microgels using a simple pipet tip-based device

Thuy P.T. Nguyen, Fanyi Li, Brendan Hung, Vinh Xuan Truong, Helmut Thissen, John S. Forsythe, Jessica E. Frith

Materials Science & Engineering

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

Microgels are microscale particles of hydrogel that can be laden with cells and used to create macroporous tissue constructs. Their ability to support cell-ECM and cell-cell interactions, along with the high levels of nutrient and metabolite exchange facilitated by their high surface area-to-volume ratio, means that they are attracting increasing attention for a variety of tissue regeneration applications. Here, we present methods for fabricating and modifying the structure of microfluidic devices using commonly available laboratory consumables including pipet tips and PTFE and silicon tubing to produce microgels. Different microfluidic devices realized the controlled generation of a wide size range (130-800 μm) of microgels for cell encapsulation. Subsequently, we describe the process of encapsulating mesenchymal stromal cells in microgels formed by photo-cross-linking of gelatin-norbornene and PEG dithiol. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easily assembled in most laboratories to effectively produce cell-laden microgels for various applications in tissue engineering.

Original language	English
Pages (from-to)	6024–6033
Number of pages	10
Journal	ACS Biomaterials Science & Engineering
Volume	9
Issue number	11
DOIs	https://doi.org/10.1021/acsbiomaterials.3c00676
Publication status	Published - 13 Nov 2023

Keywords

cell encapsulation
hydrogel
microfluidic
microgel

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10.1021/acsbiomaterials.3c00676

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title = "Cell microencapsulation within gelatin-PEG microgels using a simple pipet tip-based device",

abstract = "Microgels are microscale particles of hydrogel that can be laden with cells and used to create macroporous tissue constructs. Their ability to support cell-ECM and cell-cell interactions, along with the high levels of nutrient and metabolite exchange facilitated by their high surface area-to-volume ratio, means that they are attracting increasing attention for a variety of tissue regeneration applications. Here, we present methods for fabricating and modifying the structure of microfluidic devices using commonly available laboratory consumables including pipet tips and PTFE and silicon tubing to produce microgels. Different microfluidic devices realized the controlled generation of a wide size range (130-800 μm) of microgels for cell encapsulation. Subsequently, we describe the process of encapsulating mesenchymal stromal cells in microgels formed by photo-cross-linking of gelatin-norbornene and PEG dithiol. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easily assembled in most laboratories to effectively produce cell-laden microgels for various applications in tissue engineering.",

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author = "Nguyen, \{Thuy P.T.\} and Fanyi Li and Brendan Hung and Truong, \{Vinh Xuan\} and Helmut Thissen and Forsythe, \{John S.\} and Frith, \{Jessica E.\}",

note = "Funding Information: TPTN was supported by Monash Graduate Scholarship, Monash International Tuition Scholarship and CSIRO Postgraduate Scholarship. The Commonwealth Scientific and Industrial Research Organization (CSIRO) is acknowledged for providing a CERC Postdoctoral Fellowship to F.L. J.E.F. and J.S.F. are members of the ARC Training Centre for Cell and Tissue Engineering Technologies (IC190100026). The authors acknowledge Monash Engineering PC2 Facility for the provision of instrumentation, training and technical support. Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

year = "2023",

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doi = "10.1021/acsbiomaterials.3c00676",

language = "English",

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AU - Nguyen, Thuy P.T.

AU - Li, Fanyi

AU - Hung, Brendan

AU - Truong, Vinh Xuan

AU - Thissen, Helmut

AU - Forsythe, John S.

AU - Frith, Jessica E.

N1 - Funding Information: TPTN was supported by Monash Graduate Scholarship, Monash International Tuition Scholarship and CSIRO Postgraduate Scholarship. The Commonwealth Scientific and Industrial Research Organization (CSIRO) is acknowledged for providing a CERC Postdoctoral Fellowship to F.L. J.E.F. and J.S.F. are members of the ARC Training Centre for Cell and Tissue Engineering Technologies (IC190100026). The authors acknowledge Monash Engineering PC2 Facility for the provision of instrumentation, training and technical support. Publisher Copyright: © 2023 American Chemical Society.

PY - 2023/11/13

Y1 - 2023/11/13

N2 - Microgels are microscale particles of hydrogel that can be laden with cells and used to create macroporous tissue constructs. Their ability to support cell-ECM and cell-cell interactions, along with the high levels of nutrient and metabolite exchange facilitated by their high surface area-to-volume ratio, means that they are attracting increasing attention for a variety of tissue regeneration applications. Here, we present methods for fabricating and modifying the structure of microfluidic devices using commonly available laboratory consumables including pipet tips and PTFE and silicon tubing to produce microgels. Different microfluidic devices realized the controlled generation of a wide size range (130-800 μm) of microgels for cell encapsulation. Subsequently, we describe the process of encapsulating mesenchymal stromal cells in microgels formed by photo-cross-linking of gelatin-norbornene and PEG dithiol. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easily assembled in most laboratories to effectively produce cell-laden microgels for various applications in tissue engineering.

AB - Microgels are microscale particles of hydrogel that can be laden with cells and used to create macroporous tissue constructs. Their ability to support cell-ECM and cell-cell interactions, along with the high levels of nutrient and metabolite exchange facilitated by their high surface area-to-volume ratio, means that they are attracting increasing attention for a variety of tissue regeneration applications. Here, we present methods for fabricating and modifying the structure of microfluidic devices using commonly available laboratory consumables including pipet tips and PTFE and silicon tubing to produce microgels. Different microfluidic devices realized the controlled generation of a wide size range (130-800 μm) of microgels for cell encapsulation. Subsequently, we describe the process of encapsulating mesenchymal stromal cells in microgels formed by photo-cross-linking of gelatin-norbornene and PEG dithiol. The introduced pipet-based chip offers simplicity, tunability, and versatility, making it easily assembled in most laboratories to effectively produce cell-laden microgels for various applications in tissue engineering.

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KW - microfluidic

KW - microgel

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Cell microencapsulation within gelatin-PEG microgels using a simple pipet tip-based device

Abstract

Keywords

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ARC Training Centre for Cell and Tissue Engineering Technologies

Cite this