C/EBPδ and C/EBPγ bind the CCAAT-box in the human β-globin promoter and modulate the activity of the CACC-box binding protein, EKLF

Christopher T. Gordon, Vanessa J. Fox, Suzana Najdovska, Andrew C. Perkins

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12 Citations (Scopus)

Abstract

Developmental- and tissue-specific expression of globin genes is mediated by a few key elements within the proximal promoter of each gene. DNA-binding assays previously identified NF-Y, GATA-1, C/EBPβ and C/EBPγ as candidate regulators of β-globin transcription via the CCAAT-box, a promoter element situated between CACC- and TATA-boxes. We have identified C/EBPδ as an additional β-globin CCAAT-box binding protein. In reporter assays, we show that C/EBPδ can co-operate with EKLF, a CACC-box binding protein, to activate the β-globin promoter, whereas C/EBPγ inhibits the transcriptional activity of EKLF in this assay.

Original languageEnglish
Pages (from-to)74-80
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1729
Issue number1
DOIs
Publication statusPublished - 25 May 2005
Externally publishedYes

Keywords

  • β-globin promoter
  • C/EBPγ
  • C/EBPδ
  • CCAAT-box
  • EKLF
  • Luciferase assay

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