CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation

Kim L Good-Jacobson, Eunice Song, Shannon M Anderson, Arlene H Sharpe, Mark J Shlomchik

Research output: Contribution to journalArticleResearchpeer-review

51 Citations (Scopus)

Abstract

Germinal center (GC) B cells and T follicular helper (T(FH)) cells interact in the production of high-affinity long-lived plasma cells (PCs) and memory B cells, although the mechanisms regulating the formation of these long-lived populations remain unclear. Because CD80 is one of the few markers shared by human and murine memory B cells, we investigated its role in the development of GCs, memory cells, and PCs. In CD80-deficient mice, fewer long-lived PCs were generated upon immunization compared with that in B6 controls. In concert, the absence of CD80 resulted in an increase in apoptotic GC B cells during the contraction phase of the GC. CD80(-/-) mice had fewer T(FH) cells compared with that of B6, and residual T(FH) cells failed to mature, with decreased ICOS and PD-1 expression and decreased synthesis of IL-21 mRNA. Mixed bone marrow chimeras demonstrated a B cell-intrinsic requirement for CD80 expression for normal T(FH) cell and PC development. Therefore, B cell expression of CD80 plays a critical role in regulating B-T interactions in both early and late GC responses. This, in turn, results in impaired ability to produce long-lived PCs. These data provide new insights into the development of GCs and Ab-forming cells and the functions of CD80 in humoral immunity.
Original languageEnglish
Pages (from-to)4217 - 4225
Number of pages9
JournalJournal of Immunology
Volume188
Issue number9
DOIs
Publication statusPublished - 2012
Externally publishedYes

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