CD24 tracks divergent pluripotent states in mouse and human cells

Nika Shakiba, Carl A. White, Yonatan Y. Lipsitz, Ayako Yachie-Kinoshita, Peter D. Tonge, Samer M.I. Hussein, Mira C. Puri, Judith Elbaz, James Morrissey-Scoot, Mira Li, Javier Munoz, Marco Benevento, Ian M. Rogers, Jacob H. Hanna, Albert J.R. Heck, Bernd Wollscheid, Andras Nagy, Peter W. Zandstra

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Original languageEnglish
Article number7329
Number of pages11
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 16 Jun 2015
Externally publishedYes

Keywords

  • cell signalling
  • membrane proteins
  • pluripotent stem cells
  • reprogramming

Cite this

Shakiba, N., White, C. A., Lipsitz, Y. Y., Yachie-Kinoshita, A., Tonge, P. D., Hussein, S. M. I., ... Zandstra, P. W. (2015). CD24 tracks divergent pluripotent states in mouse and human cells. Nature Communications, 6, [7329]. https://doi.org/10.1038/ncomms8329
Shakiba, Nika ; White, Carl A. ; Lipsitz, Yonatan Y. ; Yachie-Kinoshita, Ayako ; Tonge, Peter D. ; Hussein, Samer M.I. ; Puri, Mira C. ; Elbaz, Judith ; Morrissey-Scoot, James ; Li, Mira ; Munoz, Javier ; Benevento, Marco ; Rogers, Ian M. ; Hanna, Jacob H. ; Heck, Albert J.R. ; Wollscheid, Bernd ; Nagy, Andras ; Zandstra, Peter W. / CD24 tracks divergent pluripotent states in mouse and human cells. In: Nature Communications. 2015 ; Vol. 6.
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Shakiba, N, White, CA, Lipsitz, YY, Yachie-Kinoshita, A, Tonge, PD, Hussein, SMI, Puri, MC, Elbaz, J, Morrissey-Scoot, J, Li, M, Munoz, J, Benevento, M, Rogers, IM, Hanna, JH, Heck, AJR, Wollscheid, B, Nagy, A & Zandstra, PW 2015, 'CD24 tracks divergent pluripotent states in mouse and human cells' Nature Communications, vol. 6, 7329. https://doi.org/10.1038/ncomms8329

CD24 tracks divergent pluripotent states in mouse and human cells. / Shakiba, Nika; White, Carl A.; Lipsitz, Yonatan Y.; Yachie-Kinoshita, Ayako; Tonge, Peter D.; Hussein, Samer M.I.; Puri, Mira C.; Elbaz, Judith; Morrissey-Scoot, James; Li, Mira; Munoz, Javier; Benevento, Marco; Rogers, Ian M.; Hanna, Jacob H.; Heck, Albert J.R.; Wollscheid, Bernd; Nagy, Andras; Zandstra, Peter W.

In: Nature Communications, Vol. 6, 7329, 16.06.2015.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Shakiba, Nika

AU - White, Carl A.

AU - Lipsitz, Yonatan Y.

AU - Yachie-Kinoshita, Ayako

AU - Tonge, Peter D.

AU - Hussein, Samer M.I.

AU - Puri, Mira C.

AU - Elbaz, Judith

AU - Morrissey-Scoot, James

AU - Li, Mira

AU - Munoz, Javier

AU - Benevento, Marco

AU - Rogers, Ian M.

AU - Hanna, Jacob H.

AU - Heck, Albert J.R.

AU - Wollscheid, Bernd

AU - Nagy, Andras

AU - Zandstra, Peter W.

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AB - Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

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JO - Nature Communications

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Shakiba N, White CA, Lipsitz YY, Yachie-Kinoshita A, Tonge PD, Hussein SMI et al. CD24 tracks divergent pluripotent states in mouse and human cells. Nature Communications. 2015 Jun 16;6. 7329. https://doi.org/10.1038/ncomms8329