CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

Rhys J.P. Skelton, Bevin Brady, Suhail Khoja, Debashis Sahoo, James Engel, Deevina Arasaratnam, Kholoud K. Saleh, Oscar J. Abilez, Peng Zhao, Edouard G. Stanley, Andrew G. Elefanty, Murray Kwon, David A. Elliott, Reza Ardehali

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26 Citations (Scopus)

Abstract

The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.

Original languageEnglish
Pages (from-to)95-108
Number of pages14
JournalStem Cell Reports
Volume6
Issue number1
DOIs
Publication statusPublished - 12 Jan 2016
Externally publishedYes

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