CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells

Benjamin J. Marsland, Patrick Bättig, Monika Bauer, Christiane Ruedl, Ute Lässing, Roger R. Beerli, Klaus Dietmeier, Lidia Ivanova, Thomas Pfister, Lorenz Vogt, Hideki Nakano, Chiara Nembrini, Philippe Saudan, Manfred Kopf, Martin F. Bachmann

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Dendritic cells (DCs) are key instigators of adaptive immune responses. Using an alphaviral expression cloning technology, we have identified the chemokine CCL19 as a potent inducer of T cell proliferation in a DC-T cell coculture system. Subsequent studies showed that CCL19 enhanced T cell proliferation by inducing maturation of DCs, resulting in upregulation of costimulatory molecules and the production of proinflammatory cytokines. Moreover, CCL19 programmed DCs for the induction of T helper type (Th) 1 rather than Th2 responses. Importantly, only activated DCs that migrated from the periphery to draining lymph nodes, but not resting steady-state DCs residing within lymph nodes, expressed high levels of CCR7 in vivo and responded to CCL19 with the production of proinflammatory cytokines. Migrating DCs isolated from mice genetically deficient in CCL19 and CCL21 (plt/plt) presented an only partially mature phenotype, highlighting the importance of these chemokines for full DC maturation in vivo. Our findings indicate that CCL19 and CCL21 are potent natural adjuvants for terminal activation of DCs and suggest that chemokines not only orchestrate DC migration but also regulate their immunogenic potential for the induction of T cell responses.

Original languageEnglish
Pages (from-to)493-505
Number of pages13
JournalImmunity
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Jan 2005
Externally publishedYes

Cite this

Marsland, B. J., Bättig, P., Bauer, M., Ruedl, C., Lässing, U., Beerli, R. R., ... Bachmann, M. F. (2005). CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells. Immunity, 22(4), 493-505. https://doi.org/10.1016/j.immuni.2005.02.010
Marsland, Benjamin J. ; Bättig, Patrick ; Bauer, Monika ; Ruedl, Christiane ; Lässing, Ute ; Beerli, Roger R. ; Dietmeier, Klaus ; Ivanova, Lidia ; Pfister, Thomas ; Vogt, Lorenz ; Nakano, Hideki ; Nembrini, Chiara ; Saudan, Philippe ; Kopf, Manfred ; Bachmann, Martin F. / CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells. In: Immunity. 2005 ; Vol. 22, No. 4. pp. 493-505.
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abstract = "Dendritic cells (DCs) are key instigators of adaptive immune responses. Using an alphaviral expression cloning technology, we have identified the chemokine CCL19 as a potent inducer of T cell proliferation in a DC-T cell coculture system. Subsequent studies showed that CCL19 enhanced T cell proliferation by inducing maturation of DCs, resulting in upregulation of costimulatory molecules and the production of proinflammatory cytokines. Moreover, CCL19 programmed DCs for the induction of T helper type (Th) 1 rather than Th2 responses. Importantly, only activated DCs that migrated from the periphery to draining lymph nodes, but not resting steady-state DCs residing within lymph nodes, expressed high levels of CCR7 in vivo and responded to CCL19 with the production of proinflammatory cytokines. Migrating DCs isolated from mice genetically deficient in CCL19 and CCL21 (plt/plt) presented an only partially mature phenotype, highlighting the importance of these chemokines for full DC maturation in vivo. Our findings indicate that CCL19 and CCL21 are potent natural adjuvants for terminal activation of DCs and suggest that chemokines not only orchestrate DC migration but also regulate their immunogenic potential for the induction of T cell responses.",
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Marsland, BJ, Bättig, P, Bauer, M, Ruedl, C, Lässing, U, Beerli, RR, Dietmeier, K, Ivanova, L, Pfister, T, Vogt, L, Nakano, H, Nembrini, C, Saudan, P, Kopf, M & Bachmann, MF 2005, 'CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells' Immunity, vol. 22, no. 4, pp. 493-505. https://doi.org/10.1016/j.immuni.2005.02.010

CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells. / Marsland, Benjamin J.; Bättig, Patrick; Bauer, Monika; Ruedl, Christiane; Lässing, Ute; Beerli, Roger R.; Dietmeier, Klaus; Ivanova, Lidia; Pfister, Thomas; Vogt, Lorenz; Nakano, Hideki; Nembrini, Chiara; Saudan, Philippe; Kopf, Manfred; Bachmann, Martin F.

In: Immunity, Vol. 22, No. 4, 01.01.2005, p. 493-505.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - CCL19 and CCL21 induce a potent proinflammatory differentiation program in licensed dendritic cells

AU - Marsland, Benjamin J.

AU - Bättig, Patrick

AU - Bauer, Monika

AU - Ruedl, Christiane

AU - Lässing, Ute

AU - Beerli, Roger R.

AU - Dietmeier, Klaus

AU - Ivanova, Lidia

AU - Pfister, Thomas

AU - Vogt, Lorenz

AU - Nakano, Hideki

AU - Nembrini, Chiara

AU - Saudan, Philippe

AU - Kopf, Manfred

AU - Bachmann, Martin F.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Dendritic cells (DCs) are key instigators of adaptive immune responses. Using an alphaviral expression cloning technology, we have identified the chemokine CCL19 as a potent inducer of T cell proliferation in a DC-T cell coculture system. Subsequent studies showed that CCL19 enhanced T cell proliferation by inducing maturation of DCs, resulting in upregulation of costimulatory molecules and the production of proinflammatory cytokines. Moreover, CCL19 programmed DCs for the induction of T helper type (Th) 1 rather than Th2 responses. Importantly, only activated DCs that migrated from the periphery to draining lymph nodes, but not resting steady-state DCs residing within lymph nodes, expressed high levels of CCR7 in vivo and responded to CCL19 with the production of proinflammatory cytokines. Migrating DCs isolated from mice genetically deficient in CCL19 and CCL21 (plt/plt) presented an only partially mature phenotype, highlighting the importance of these chemokines for full DC maturation in vivo. Our findings indicate that CCL19 and CCL21 are potent natural adjuvants for terminal activation of DCs and suggest that chemokines not only orchestrate DC migration but also regulate their immunogenic potential for the induction of T cell responses.

AB - Dendritic cells (DCs) are key instigators of adaptive immune responses. Using an alphaviral expression cloning technology, we have identified the chemokine CCL19 as a potent inducer of T cell proliferation in a DC-T cell coculture system. Subsequent studies showed that CCL19 enhanced T cell proliferation by inducing maturation of DCs, resulting in upregulation of costimulatory molecules and the production of proinflammatory cytokines. Moreover, CCL19 programmed DCs for the induction of T helper type (Th) 1 rather than Th2 responses. Importantly, only activated DCs that migrated from the periphery to draining lymph nodes, but not resting steady-state DCs residing within lymph nodes, expressed high levels of CCR7 in vivo and responded to CCL19 with the production of proinflammatory cytokines. Migrating DCs isolated from mice genetically deficient in CCL19 and CCL21 (plt/plt) presented an only partially mature phenotype, highlighting the importance of these chemokines for full DC maturation in vivo. Our findings indicate that CCL19 and CCL21 are potent natural adjuvants for terminal activation of DCs and suggest that chemokines not only orchestrate DC migration but also regulate their immunogenic potential for the induction of T cell responses.

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U2 - 10.1016/j.immuni.2005.02.010

DO - 10.1016/j.immuni.2005.02.010

M3 - Article

VL - 22

SP - 493

EP - 505

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 4

ER -