Caveolin-1 is required for lateral line neuromast and notochord development

Susan J. Nixon, Adrian Carter, Jeremy Wegner, Charles Ferguson, Matthias Floetenmeyer, Jamie Riches, Brian Key, Monte Westerfield, Robert G. Parton

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41 Citations (Scopus)

Abstract

Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory organs of the lateral line, the neuromasts and in the notochord where it overlaps with expression of caveolin-3. Morpholino-mediated downregulation of Cav1α caused a dramatic inhibition of neuromast formation. Detailed ultrastructural analysis, including electron tomography of the notochord, revealed that the central regions of the notochord has the highest density of caveolae of any embryonic tissue comparable to the highest density observed in any vertebrate tissue. In addition, Cav1α downregulation caused disruption of the notochord, an effect that was enhanced further by Cav3 knockdown. These results indicate an essential role for caveolin and caveolae in this vital structural and signalling component of the embryo.

Original languageEnglish
Pages (from-to)2151-2161
Number of pages11
JournalJournal of Cell Science
Volume120
Issue number13
DOIs
Publication statusPublished - 1 Jul 2007

Keywords

  • Caveolae
  • Caveolin-1
  • Neuromast
  • Notochord
  • Zebrafish

Cite this

Nixon, S. J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J., Key, B., Westerfield, M., & Parton, R. G. (2007). Caveolin-1 is required for lateral line neuromast and notochord development. Journal of Cell Science, 120(13), 2151-2161. https://doi.org/10.1242/jcs.003830