Cationic poly(2-oxazoline) hydrogels for reversible DNA binding

Matthias Hartlieb; David Pretzel; Kristian Kempe; Carolin Fritzsche; Renzo M. Paulus; Michael Gottschaldt; Ulrich S. Schubert

doi:10.1039/c3sm00114h

Cationic poly(2-oxazoline) hydrogels for reversible DNA binding

Matthias Hartlieb, David Pretzel, Kristian Kempe, Carolin Fritzsche, Renzo M. Paulus, Michael Gottschaldt, Ulrich S. Schubert

Research output: Contribution to journal › Article › Research › peer-review

50 Citations (Scopus)

Abstract

A new 2-oxazoline monomer with a Boc-protected amino group in the side chain (BocOx) was synthesized. Homopolymerization as well as copolymerization with 2-ethyl-2-oxazoline (EtOx) revealed a pseudo first order kinetic. A series of homopolymers was synthesized, deprotected and characterized regarding their structure and thermal properties. The copolymerization with EtOx yielded a series of water soluble polymers with varying amino contents. After deprotection it was shown by the ethidium bromide assay that these polymers were able to form complexes with DNA. Treatment with epichlorohydrin leads to the formation of hydrogels. The swelling properties of the gels were investigated and it could be demonstrated that also the polymeric scaffolds were able to immobilize DNA from aqueous solution. Furthermore, the release of the DNA was accomplished using heparin.

Original language	English
Pages (from-to)	4693-4704
Number of pages	12
Journal	Soft Matter
Volume	9
Issue number	18
DOIs	https://doi.org/10.1039/c3sm00114h
Publication status	Published - 14 May 2013
Externally published	Yes

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abstract = "A new 2-oxazoline monomer with a Boc-protected amino group in the side chain (BocOx) was synthesized. Homopolymerization as well as copolymerization with 2-ethyl-2-oxazoline (EtOx) revealed a pseudo first order kinetic. A series of homopolymers was synthesized, deprotected and characterized regarding their structure and thermal properties. The copolymerization with EtOx yielded a series of water soluble polymers with varying amino contents. After deprotection it was shown by the ethidium bromide assay that these polymers were able to form complexes with DNA. Treatment with epichlorohydrin leads to the formation of hydrogels. The swelling properties of the gels were investigated and it could be demonstrated that also the polymeric scaffolds were able to immobilize DNA from aqueous solution. Furthermore, the release of the DNA was accomplished using heparin.",

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AU - Hartlieb, Matthias

AU - Pretzel, David

AU - Kempe, Kristian

AU - Fritzsche, Carolin

AU - Paulus, Renzo M.

AU - Gottschaldt, Michael

AU - Schubert, Ulrich S.

PY - 2013/5/14

Y1 - 2013/5/14

N2 - A new 2-oxazoline monomer with a Boc-protected amino group in the side chain (BocOx) was synthesized. Homopolymerization as well as copolymerization with 2-ethyl-2-oxazoline (EtOx) revealed a pseudo first order kinetic. A series of homopolymers was synthesized, deprotected and characterized regarding their structure and thermal properties. The copolymerization with EtOx yielded a series of water soluble polymers with varying amino contents. After deprotection it was shown by the ethidium bromide assay that these polymers were able to form complexes with DNA. Treatment with epichlorohydrin leads to the formation of hydrogels. The swelling properties of the gels were investigated and it could be demonstrated that also the polymeric scaffolds were able to immobilize DNA from aqueous solution. Furthermore, the release of the DNA was accomplished using heparin.

AB - A new 2-oxazoline monomer with a Boc-protected amino group in the side chain (BocOx) was synthesized. Homopolymerization as well as copolymerization with 2-ethyl-2-oxazoline (EtOx) revealed a pseudo first order kinetic. A series of homopolymers was synthesized, deprotected and characterized regarding their structure and thermal properties. The copolymerization with EtOx yielded a series of water soluble polymers with varying amino contents. After deprotection it was shown by the ethidium bromide assay that these polymers were able to form complexes with DNA. Treatment with epichlorohydrin leads to the formation of hydrogels. The swelling properties of the gels were investigated and it could be demonstrated that also the polymeric scaffolds were able to immobilize DNA from aqueous solution. Furthermore, the release of the DNA was accomplished using heparin.

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Cationic poly(2-oxazoline) hydrogels for reversible DNA binding

Abstract

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