Catechin Glucosides: Occurrence, synthesis, and stability

Thomas Raab, Denis Barron, Francia Arce Vera, Vanessa Crespy, Manuel Oliveira, Gary Williamson

Research output: Contribution to journalArticleResearchpeer-review

50 Citations (Scopus)

Abstract

Catechins are flavonoids with suggested health benefits, but are unstable during storage, processing and, after ingestion, during gut transit. We hypothesized that catechin glucosidos, which occur in various plants, could be more stable than unsubstituted catechin, and additionally be deglucosylated in the gut and so act to deliver catechin in a form able to be absorbed. (+)-Catechin O-glucosides from various sources have been used in the course of this investigation. (+)-Catechin 3'-O-β-D-glucopyranoside (C3'G), (+)-catechin 5-O-β-D-glucopyranoside (C5G), and (+)-catechin 3-O-β-D-glucopyranoside (C3G) were chemically synthesized. (+)-Catechin 4'-O-β-D-glucopyranoside (C4'G) and (+)-catechin 7-O-β-D-glucopyranoside (C7G) were prepared enzymically using preparations from lentil and barley. In general, but with some exceptions, the (+)-catechin glucosidos were more stable between pH 4 and 8 than (+)-catechin, with C3'G exhibiting greatest stability. The intestinal metabolism of (+)-catechin and all (+)-catechin glucosides in the gut was determined by perfusion of rat intestine in vivo. C3'G and C5G were extensively deglycosylated in the gut, and C3'G showed greatest apparent "absorption" as calculated by the difference between effluent and influent. The results show the potential of catechin glucosides, especially C3'G, as more stable prescursors of catechin.

Original languageEnglish
Pages (from-to)2138-2149
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number4
DOIs
Publication statusPublished - 24 Feb 2010
Externally publishedYes

Keywords

  • Absorption
  • Catechin
  • Chemical synthesis
  • Flavonoid
  • Glucosldase
  • Stability

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