Catalysis product captured in lumazine synthase from the fungal pathogen Candida glabrata

Madhu Shankar, Sigurd M. Wilbanks, Yoshio Nakatani, Brian C. Monk, Joel D A Tyndall

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Candida glabrata has emerged as an important fungal pathogen with intrinsic resistance to azole drugs. The limited efficacy of and resistance to existing antifungals is driving the need to identify new drug targets. The enzyme 6,7-dimethyl-8-(d-ribityl)lumazine synthase is part of the riboflavin- biosynthesis pathway essential to fungi and bacteria and is a potential drug target for the development of broad-spectrum antifungal drugs. The X-ray crystal structure of recombinant lumazine synthase from C. glabrata was obtained at 2.24 Å resolution and revealed a dimer of homopentamers, with one in five subunits containing a product molecule from the catalytic reaction. 

Original languageEnglish
Pages (from-to)1580-1586
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume69
Issue number8
DOIs
Publication statusPublished - Aug 2013
Externally publishedYes

Keywords

  • 6,7-dimethyl-8-(d-ribityl)lumazine
  • Candida glabrata
  • fungal pathogens
  • lumazine synthase

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