Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7

Po Ki Ho, Anissa Jabbour, Paul G Ekert, Christine J Hawkins

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26 Citations (Scopus)

Abstract

Caspases are a family of cysteine proteases with roles in cytokine maturation or apoptosis. Caspase-2 was the first pro-apoptotic caspase identified, but its functions in apoptotic signal transduction are still being elucidated. This study examined the regulation of the activity of caspase-2 using recombinant proteins and a yeast-based system. Our data suggest that for human caspase-2 to be active its large and small subunits must be separated. For maximal activity its prodomain must also be removed. Consistent with its proposed identity as an upstream caspase, caspase-2 could provoke the activation of caspase-7. Caspase-2 was not subject to inhibition by members of the IAP family of apoptosis inhibitors.
Original languageEnglish
Pages (from-to)1401 - 1414
Number of pages14
JournalFEBS Journal
Volume272
Issue number6
DOIs
Publication statusPublished - 2005
Externally publishedYes

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