Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 77 - 86 |
| Number of pages | 10 |
| Journal | Advanced Healthcare Materials |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 |
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Cardiogenesis of embryonic stem cells with liquid marble micro-bioreactor. / Sarvi, Fatemeh; Jain, Kanika; Akbarzadeh Arbatan, Tina; Verma, Paul John; Hourigan, Kerry; Thompson, Mark Christopher; Shen, Wei; Chan, Pui Yik Peggy.
In: Advanced Healthcare Materials, Vol. 4, No. 1, 2015, p. 77 - 86.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Cardiogenesis of embryonic stem cells with liquid marble micro-bioreactor
AU - Sarvi, Fatemeh
AU - Jain, Kanika
AU - Akbarzadeh Arbatan, Tina
AU - Verma, Paul John
AU - Hourigan, Kerry
AU - Thompson, Mark Christopher
AU - Shen, Wei
AU - Chan, Pui Yik Peggy
PY - 2015
Y1 - 2015
N2 - A liquid marble micro-bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2-ESC) suspension onto a polytetrafluoroethylene (PTFE) particle bed. The Oct4B2-ESC aggregates to form embryoid bodies (EBs) with relatively uniform size and shape in a liquid marble within 3 d. For the first time, the feasibility of differentiating ESC into cardiac lineages within liquid marbles is being investigated. Without the addition of growth factors, suspended EBs from liquid marbles express various precardiac mesoderm markers including Flk-1, Gata4, and Nkx2.5. Some of the suspended EBs exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac mesoderm differentiation. Some of the EBs are subsequently plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiac markers atrial myosin light chain 2a (MLC2a) and ventricular myosin light chain (MLC2v), and the cardiac structural marker a-actinin. More than 60 of the plated EBs exhibit spontaneous contraction and express mature cardiomyocyte marker cardiac troponin T (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. Together, these results demonstrate that the liquid-marble technique is an easily employed, cost effective, and efficient approach to generate EBs and facilitating their cardiogenesis.
AB - A liquid marble micro-bioreactor is prepared by placing a drop of murine embryonic stem cell (ESC) (Oct4B2-ESC) suspension onto a polytetrafluoroethylene (PTFE) particle bed. The Oct4B2-ESC aggregates to form embryoid bodies (EBs) with relatively uniform size and shape in a liquid marble within 3 d. For the first time, the feasibility of differentiating ESC into cardiac lineages within liquid marbles is being investigated. Without the addition of growth factors, suspended EBs from liquid marbles express various precardiac mesoderm markers including Flk-1, Gata4, and Nkx2.5. Some of the suspended EBs exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac mesoderm differentiation. Some of the EBs are subsequently plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiac markers atrial myosin light chain 2a (MLC2a) and ventricular myosin light chain (MLC2v), and the cardiac structural marker a-actinin. More than 60 of the plated EBs exhibit spontaneous contraction and express mature cardiomyocyte marker cardiac troponin T (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. Together, these results demonstrate that the liquid-marble technique is an easily employed, cost effective, and efficient approach to generate EBs and facilitating their cardiogenesis.
UR - http://onlinelibrary.wiley.com/doi/10.1002/adhm.201400138/pdf
U2 - 10.1002/adhm.201400138
DO - 10.1002/adhm.201400138
M3 - Article
VL - 4
SP - 77
EP - 86
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
SN - 2192-2640
IS - 1
ER -