Cardio-protective effects of combined l-arginine and insulin: mechanism and therapeutic actions in myocardial ischemia-reperfusion injury

Kylie M Venardos, Niwanthi W Rajapakse, David Williams, Louise See Hoe, Jason N Peart, David M Kaye

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Reduced nitric oxide (NO) bioavailability plays a central role in the pathogenesis of myocardial ischemia-reperfusion injury (I-R), and reduced l-arginine transport via cationic amino acid transporter-1 is a key contributor to the reduced NO levels. Insulin can increase NO levels by increasing the transport of its substrate l-arginine but insulin alone exerts minimal cardiac protection in I-R. We hypothesised that combined insulin and l-arginine may provide cardioprotective effects in the setting of myocardial I-R. The effect of supplemental insulin, l-arginine and the combination was examined in cardiomyocytes exposed to hypoxia/reoxygenation and in isolated perfused mouse hearts undergoing ischemia/reperfusion. When compared to controls, cardiomyocytes treated upon reoxygenation with 1nM insulin+1mM l-arginine exhibited significant (all P
Original languageEnglish
Pages (from-to)64 - 70
Number of pages7
JournalEuropean Journal of Pharmacology
Volume769
DOIs
Publication statusPublished - 2015

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