Carbonate apatite nanoparticles carry siRNA(S) targeting growth factor receptor genes egfr1 and erbb2 to regress mouse breast tumor

Snigdha Tiash, Nur Izyani Binti Kamaruzman, Ezharul Hoque Chowdhury

    Research output: Contribution to journalArticleResearchpeer-review

    14 Citations (Scopus)

    Abstract

    Cancer cells lose their control on cell cycle by numerous genetic and epigenetic alterations. In a tumor, these cells highly express growth factor receptors (GFRs), eliciting growth, and cell division. Among the GFRs, epidermal growth factor receptor-1 (EGFR1) (Her1/ERBB1) and epidermal growth factor receptor- 2 (EGFR2) (Her2/ERBB2) from epidermal growth factor (EGF) family and insulin-like growth factor-1 receptor (IGF1R) are highly expressed on breast cancer cells, thus contributing to the aggressive growth and invasiveness, have been focused in this study. Moreover, overexpression of these receptors is related to suppression of cell death and conferring resistance against the classical drugs used to treat cancer nowadays. Therefore, silencing of these GFRs-encoding genes by using selective small interfering RNAs (siRNAs) could be a powerful approach to treat breast cancer. The inorganic pH sensitive carbonate apatite nanoparticles (NPs) were used as a nano-carrier to deliver siRNA(s) against single or multiple GFR genes in breast cancer cells as well as in a mouse model of breast carcinoma. Silencing of egfr1 and erbb2 simultaneously led to a reduction in cell viability with an increase in cell death signal in the cancer cells and regression of tumor growth in vivo.

    Original languageEnglish
    Pages (from-to)1721-1730
    Number of pages10
    JournalDrug Delivery
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - 9 Nov 2017

    Keywords

    • Breast cancer
    • Carbonate apatite nanoparticles (NPs)
    • Gene silencing
    • Growth factor receptors
    • SiRNA
    • Tumor regression

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