Carbohydrate-independent recognition of collagens by the macrophage mannose receptor

Luisa Martinez-Pomares, Dirk Wienke, Richard Stallion, Emma J. McKenzie, James N. Arnold, James Harris, Eamon McGreal, Robert B. Sim, Clare M. Isacke, Siamon Gordon

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Monnose receptor (MR) is the best characterised member of a family of four endocytic molecules that share a common domain structure; a cysteine-rich (CR) domain, a fibronectin-type II (FNII) domain and tandemly arranged C-type lectin-like domains (CTLD, eight in the case of MR). Two distinct lectin activities have been described for MR. The CR domain recognises sulphated carbohydrates while the CTLD mediate binding to mannose, fucose or N-acetylglucosamine. FNII domains are known to be important for collagen binding and this has been studied in the context of two members of the MR family, Endo180 and the phospholipase A2 receptor. Here, we have investigated whether the broad and effective lectin activity mediated by the CR domain and CTLD of MR is favoured to the detriment of FNII-mediated interaction (s). We show that MR is able to bind and internalise collagen in a carbohydrate-independent manner and that MR deficient macrophages have a marked defect in collagen IV and gelatin internalisation. These data have major implications at the molecular level as there are now three distinct ligand-binding sites described for MR. Furthermore our findings extend the range of endogenous ligands recognised by MR, a molecule firmly placed at the interface between homeostasis and immunity.

Original languageEnglish
Pages (from-to)1074-1082
Number of pages9
JournalEuropean Journal of Immunology
Volume36
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Keywords

  • CD206/208
  • Endo180
  • Extracellular matrix
  • Macrophages
  • Mannhose receptor

Cite this

Martinez-Pomares, L., Wienke, D., Stallion, R., McKenzie, E. J., Arnold, J. N., Harris, J., ... Gordon, S. (2006). Carbohydrate-independent recognition of collagens by the macrophage mannose receptor. European Journal of Immunology, 36(5), 1074-1082. https://doi.org/10.1002/eji.200535685
Martinez-Pomares, Luisa ; Wienke, Dirk ; Stallion, Richard ; McKenzie, Emma J. ; Arnold, James N. ; Harris, James ; McGreal, Eamon ; Sim, Robert B. ; Isacke, Clare M. ; Gordon, Siamon. / Carbohydrate-independent recognition of collagens by the macrophage mannose receptor. In: European Journal of Immunology. 2006 ; Vol. 36, No. 5. pp. 1074-1082.
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Martinez-Pomares, L, Wienke, D, Stallion, R, McKenzie, EJ, Arnold, JN, Harris, J, McGreal, E, Sim, RB, Isacke, CM & Gordon, S 2006, 'Carbohydrate-independent recognition of collagens by the macrophage mannose receptor' European Journal of Immunology, vol. 36, no. 5, pp. 1074-1082. https://doi.org/10.1002/eji.200535685

Carbohydrate-independent recognition of collagens by the macrophage mannose receptor. / Martinez-Pomares, Luisa; Wienke, Dirk; Stallion, Richard; McKenzie, Emma J.; Arnold, James N.; Harris, James; McGreal, Eamon; Sim, Robert B.; Isacke, Clare M.; Gordon, Siamon.

In: European Journal of Immunology, Vol. 36, No. 5, 05.2006, p. 1074-1082.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Carbohydrate-independent recognition of collagens by the macrophage mannose receptor

AU - Martinez-Pomares, Luisa

AU - Wienke, Dirk

AU - Stallion, Richard

AU - McKenzie, Emma J.

AU - Arnold, James N.

AU - Harris, James

AU - McGreal, Eamon

AU - Sim, Robert B.

AU - Isacke, Clare M.

AU - Gordon, Siamon

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AB - Monnose receptor (MR) is the best characterised member of a family of four endocytic molecules that share a common domain structure; a cysteine-rich (CR) domain, a fibronectin-type II (FNII) domain and tandemly arranged C-type lectin-like domains (CTLD, eight in the case of MR). Two distinct lectin activities have been described for MR. The CR domain recognises sulphated carbohydrates while the CTLD mediate binding to mannose, fucose or N-acetylglucosamine. FNII domains are known to be important for collagen binding and this has been studied in the context of two members of the MR family, Endo180 and the phospholipase A2 receptor. Here, we have investigated whether the broad and effective lectin activity mediated by the CR domain and CTLD of MR is favoured to the detriment of FNII-mediated interaction (s). We show that MR is able to bind and internalise collagen in a carbohydrate-independent manner and that MR deficient macrophages have a marked defect in collagen IV and gelatin internalisation. These data have major implications at the molecular level as there are now three distinct ligand-binding sites described for MR. Furthermore our findings extend the range of endogenous ligands recognised by MR, a molecule firmly placed at the interface between homeostasis and immunity.

KW - CD206/208

KW - Endo180

KW - Extracellular matrix

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