Capturing the interactome of newly transcribed RNA

Xichen Bao, Xiangpeng Guo, Menghui Yin, Muqddas Tariq, Yiwei Lai, Shahzina Kanwal, Jiajian Zhou, Na Li, Yuan Lv, Carlos Pulido-Quetglas, Xiwei Wang, Lu Ji, Muhammad J. Khan, Xihua Zhu, Zhiwei Luo, Changwei Shao, Do Hwan Lim, Xiao Liu, Nan Li, Wei WangMinghui He, Yu Lin Liu, Carl Ward, Tong Wang, Gong Zhang, Dongye Wang, Jianhua Yang, Yiwen Chen, Chaolin Zhang, Ralf Jauch, Yun Gui Yang, Yangming Wang, Baoming Qin, Minna Liisa Anko, Andrew P. Hutchins, Hao Sun, Huating Wang, Xiang Dong Fu, Biliang Zhang, Miguel A. Esteban

Research output: Contribution to journalArticleResearchpeer-review

70 Citations (Scopus)

Abstract

We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalNature Methods
Volume15
Issue number3
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • interactome
  • RNA binding protein
  • labelling
  • proteomic analysis
  • proteomics

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