TY - JOUR
T1 - Capillary electrophoresis of methotrexate polyglutamates and its application in evaluation of γ-glutamyl hydrolase activity
AU - Waltham, Mark C.
AU - Lin, Silan
AU - Li, Wei Wei
AU - Göker, Erdem
AU - Gritsman, Helena
AU - Tong, William P.
AU - Bertino, Joseph R.
PY - 1997/2/21
Y1 - 1997/2/21
N2 - A rapid and sensitive procedure for the separation of methotrexate (MTX) polyglutamates using capillary electrophoresis (CE) is described as it applies to the in vitro assay of the enzyme γ-glutamyl hydrolase (GGH, EC 3.4.22.12). Distinct separation of MTX and polyglutamylated forms (up to glu4) is achieved within 10 min using a 75 μm I.D. capillary (50 cm, +25 kV), and enables quantitation of both reactant and enzyme products. As activity can be reliably determined using less than 5x105 eukaryotic cells, this new technique can be used to measure GGH in patient tumor samples and investigate the relationship between GGH levels and clinical MTX resistance.
AB - A rapid and sensitive procedure for the separation of methotrexate (MTX) polyglutamates using capillary electrophoresis (CE) is described as it applies to the in vitro assay of the enzyme γ-glutamyl hydrolase (GGH, EC 3.4.22.12). Distinct separation of MTX and polyglutamylated forms (up to glu4) is achieved within 10 min using a 75 μm I.D. capillary (50 cm, +25 kV), and enables quantitation of both reactant and enzyme products. As activity can be reliably determined using less than 5x105 eukaryotic cells, this new technique can be used to measure GGH in patient tumor samples and investigate the relationship between GGH levels and clinical MTX resistance.
KW - γ-Glutamyl hydrolase
KW - 4-Amino-10-methylpteroylglutamate
KW - Methotrexate polyglutamates
UR - http://www.scopus.com/inward/record.url?scp=0031581908&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(96)00350-7
DO - 10.1016/S0378-4347(96)00350-7
M3 - Article
C2 - 9080326
AN - SCOPUS:0031581908
SN - 1572-6495
VL - 689
SP - 387
EP - 392
JO - Journal of Chromatography B - Biomedical Applications
JF - Journal of Chromatography B - Biomedical Applications
IS - 2
ER -