Can α- and β-Alanine Containing Peptides Be Distinguished Based on the CID Spectra of Their Protonated Ions?

A. K Y Lam, Sri H. Ramarathinam, Anthony W. Purcell, R. A J O'Hair

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Abstract

The fragmentation reactions of isomeric dipeptides containing α- and β-alanine residues (αAla-αAla, αAla-βAla, βAla-αAla, and βAla-βAla) were studied using a combination of low-energy and energy resolved collision induced dissociation (CID). Each dipeptide gave a series of different fragment ions, allowing for differentiation. For example, peptides containing an N-terminal β-Ala residue yield a diagnostic imine loss, while lactam ions at m/z 72 are unique to peptides containing β-Ala residues. In addition, MS3 experiments were performed. Structure-specific fragmentation reactions were observed for y1 ions, which help identify the C-terminal residue. The MS3 spectra of the b2 ions are different suggesting they are unique for each peptide. Density functional theory (DFT) calculations predict that b2 ions formed via a neighboring group attack by the amide are thermodynamically favored over those formed via neighboring group attack by the N-terminal amine. Finally, to gain further insight into the unique fragmentation chemistry of the peptides containing an N-terminal β-alanine residue, the fragmentation reactions of protonated β-Ala-NHMe were examined using a combination of experiment and DFT calculations. The relative transition-state energies involved in the four competing losses (NH3, H2O, CH3NH2, and CH2=NH) closely follow the relative abundances of these as determined via CID experiments.

Original languageEnglish
Pages (from-to)1743-1754
Number of pages12
JournalJournal of the American Society for Mass Spectrometry
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 2008

Cite this

@article{21718ecf8e844433b7adde638c997c98,
title = "Can α- and β-Alanine Containing Peptides Be Distinguished Based on the CID Spectra of Their Protonated Ions?",
abstract = "The fragmentation reactions of isomeric dipeptides containing α- and β-alanine residues (αAla-αAla, αAla-βAla, βAla-αAla, and βAla-βAla) were studied using a combination of low-energy and energy resolved collision induced dissociation (CID). Each dipeptide gave a series of different fragment ions, allowing for differentiation. For example, peptides containing an N-terminal β-Ala residue yield a diagnostic imine loss, while lactam ions at m/z 72 are unique to peptides containing β-Ala residues. In addition, MS3 experiments were performed. Structure-specific fragmentation reactions were observed for y1 ions, which help identify the C-terminal residue. The MS3 spectra of the b2 ions are different suggesting they are unique for each peptide. Density functional theory (DFT) calculations predict that b2 ions formed via a neighboring group attack by the amide are thermodynamically favored over those formed via neighboring group attack by the N-terminal amine. Finally, to gain further insight into the unique fragmentation chemistry of the peptides containing an N-terminal β-alanine residue, the fragmentation reactions of protonated β-Ala-NHMe were examined using a combination of experiment and DFT calculations. The relative transition-state energies involved in the four competing losses (NH3, H2O, CH3NH2, and CH2=NH) closely follow the relative abundances of these as determined via CID experiments.",
author = "Lam, {A. K Y} and Ramarathinam, {Sri H.} and Purcell, {Anthony W.} and O'Hair, {R. A J}",
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Can α- and β-Alanine Containing Peptides Be Distinguished Based on the CID Spectra of Their Protonated Ions? / Lam, A. K Y; Ramarathinam, Sri H.; Purcell, Anthony W.; O'Hair, R. A J.

In: Journal of the American Society for Mass Spectrometry, Vol. 19, No. 12, 12.2008, p. 1743-1754.

Research output: Contribution to journalArticleResearchpeer-review

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AU - O'Hair, R. A J

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