Calorimetry and Langmuir-Blodgett studies on the interaction of a lipophilic prodrug of LHRH with biomembrane models

Maria G. Sarpietro, Maria L. Accolla, Nancy Santoro, Friederike M. Mansfeld, Rosario Pignatello, Istvan Toth, Francesco Castelli

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6 Citations (Scopus)

Abstract

The interaction between an amphiphilic luteinizing hormone-releasing hormone (LHRH) prodrug that incorporated a lipoamino acid moiety (C12-LAA) with biological membrane models that consisted of multilamellar liposomes (MLVs) and phospholipid monolayers, was studied using Differential Scanning Calorimetry (DSC) and Langmuir-Blodgett film techniques. The effect of the prodrug C12[Q1]LHRH on the lipid layers was compared with the results obtained with the pure precursors, LHRH and C12-LAA.Conjugation of LHRH with a LAA promoiety showed to improve the peptide interaction with biomembrane models. Basing on the calorimetric findings, the LAA moiety aided the transfer of the prodrug from an aqueous solution to the biomembrane model.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalJournal of Colloid and Interface Science
Volume421
DOIs
Publication statusPublished - 1 May 2014
Externally publishedYes

Keywords

  • Calorimetry
  • DMPC
  • Drug-membrane interaction
  • DSC
  • Langmuir-Blodgett film
  • Lipoamino acids
  • Liposomes
  • Prodrug

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