@article{5ff8b0a4bfe3416c9bf63c0962471e4e,
title = "Calorie Restriction Increases the Number of Competing Stem Cells and Decreases Mutation Retention in the Intestine",
abstract = "Calorie restriction increases the number of stem cells in intestinal crypts. Bruens et al. show that this increase results in stronger stem cell competition. Consequently, mutated stem cells encounter more wild-type competitors, which decreases the chance that these mutated stem cells are retained in the intestinal epithelium.",
keywords = "calorie restriction, competition, diet, intestine, Lgr5, mutation retention, stem cells",
author = "Lotte Bruens and Ellenbroek, \{Saskia Inge Johanna\} and Suijkerbuijk, \{Saskia Jacoba Elisabeth\} and Maria Azkanaz and Hale, \{Alexander James\} and Pim Toonen and Flanagan, \{Dustin James\} and Sansom, \{Owen James\} and Snippert, \{Hugo Johannes\} and \{van Rheenen\}, Jacco",
note = "Funding Information: The authors would like to thank the staff at The Hubrecht Institute animal facility and the Netherlands Cancer Institute (NKI) animal facility and bioimaging facility for technical support and input and members of the van Rheenen laboratory for discussions. They would also like to thank Maartje Damen for help with the graphical abstract. This work was supported by the Netherlands Organization of Scientific Research ( NWO ) (Veni grant 863.15.011 to S.I.J.E. and Cancer Genomics Netherlands to J.v.R.), the European Research Council (consolidator grant 648804 to J.v.R.), Worldwide Cancer Research (grant 13-0297 to J.v.R.), the Dutch Cancer Society ( KWF ) (grant 2013-6070 to H.J.S.), and the Doctor Josef Steiner Foundation , Switzerland (to J.v.R.). This work was also supported by a Cancer Research UK core grant to the Beatson Institute ( A17196 ) and core funding to O.J.S. ( A21139 ). In particular, they would like to thank the Core Services and Advanced Technologies at the Cancer Research UK Beatson Institute. D.J.F. was funded by MRC grant MR/R017247/1 . Funding Information: The authors would like to thank the staff at The Hubrecht Institute animal facility and the Netherlands Cancer Institute (NKI) animal facility and bioimaging facility for technical support and input and members of the van Rheenen laboratory for discussions. They would also like to thank Maartje Damen for help with the graphical abstract. This work was supported by the Netherlands Organization of Scientific Research (NWO) (Veni grant 863.15.011 to S.I.J.E. and Cancer Genomics Netherlands to J.v.R.), the European Research Council (consolidator grant 648804 to J.v.R.), Worldwide Cancer Research (grant 13-0297 to J.v.R.), the Dutch Cancer Society (KWF) (grant 2013-6070 to H.J.S.), and the Doctor Josef Steiner Foundation, Switzerland (to J.v.R.). This work was also supported by a Cancer Research UK core grant to the Beatson Institute (A17196) and core funding to O.J.S. (A21139). In particular, they would like to thank the Core Services and Advanced Technologies at the Cancer Research UK Beatson Institute. D.J.F. was funded by MRC grant MR/R017247/1. L.B. H.J.S. and J.v.R. perceived the conceptual ideas and designed the experiments. L.B. S.I.J.E. H.J.S. and J.v.R. wrote the manuscript. L.B. S.I.J.E. S.J.E.S. M.A. A.J.H. and P.T. performed experiments. D.J.F. and O.J.S. facilitated the in situ hybridizations. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = jul,
day = "21",
doi = "10.1016/j.celrep.2020.107937",
language = "English",
volume = "32",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Elsevier",
number = "3",
}