Calcium inflow in cells transfected with cloned rat and porcine calcitonin receptors

David M. Findlay, Souheir Houssami, Patrick M. Sexton, Catherine L. Brady, T. John Martin, Damian E. Myers

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Ca 2+ fluxes were examined in HEK 293 cells stably expressing the rat or porcine calcitonin receptors (CTRs). Calcitonin (CT) rapidly increased cytosolic Ca 2+ ([Ca 2+ ] i ) concentrations in these cells in a manner which was sustained in the presence of extracellular Ca 2+ ([Ca 2+] e ). In cells pretreated with CT, elevation of the [Ca 2+ ] e concentration resulted in a further increase in [Ca 2+ ] i which was concentration-dependent with respect to both the concentration of CT and the increment of [Ca 2+ e . Untransfected cells, cells transfected with vector alone, and CTR-transfected cells not treated with CT, were unresponsive to [Ca 2+ ] e . The microsomal Ca 2+ -ATPase inhibitor thapsigargin was able to mimic both the acute [Ca 2+ ] i fluxes and responsiveness to [Ca 2+ ] e mediated by Cr in these cells. The CT-induced responsiveness to [Ca 2+ ] e was neither mimicked by, nor affected by, activators of the cAMP or protein kinase C pathways. Treatment of cells with pertussis toxin influenced neither the primary Ca 2+ fluxes in response to CT or thapsigargin nor the agonist-induced [Ca 2+ ] e influx. Nifedipine failed to block responses to either CT or thapsigargin. These results lead to the important conclusion that the CTR participates in receptor-activated Ca 2+ inflow, in which depletion of intracellular Ca 2+ pools leads secondarily to influx of extracellular Ca2+.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalBBA - Molecular Cell Research
Volume1265
Issue number2-3
DOIs
Publication statusPublished - 16 Mar 1995
Externally publishedYes

Keywords

  • Calcium influx
  • Cloned calcitonin receptor
  • Thapsigargin
  • Transfected HEK 293 cell

Cite this

Findlay, David M. ; Houssami, Souheir ; Sexton, Patrick M. ; Brady, Catherine L. ; Martin, T. John ; Myers, Damian E. / Calcium inflow in cells transfected with cloned rat and porcine calcitonin receptors. In: BBA - Molecular Cell Research. 1995 ; Vol. 1265, No. 2-3. pp. 213-219.
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Calcium inflow in cells transfected with cloned rat and porcine calcitonin receptors. / Findlay, David M.; Houssami, Souheir; Sexton, Patrick M.; Brady, Catherine L.; Martin, T. John; Myers, Damian E.

In: BBA - Molecular Cell Research, Vol. 1265, No. 2-3, 16.03.1995, p. 213-219.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Findlay, David M.

AU - Houssami, Souheir

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AU - Martin, T. John

AU - Myers, Damian E.

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