Calcium-dependent complex formation between PBP2 and Lytic transglycosylase SltB1 of Pseudomonas aeruginosa

Ioulia Nikolaidis, Thierry Izore, Viviana Job, Nicole Thielens, Eefjan Breukink, Andrea Dessen

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

In Gram-negative bacteria, the bacterial cell wall biosynthetic mechanism requires the coordinated action of enzymes and structural proteins located in the cytoplasm, within the membrane, and in the periplasm of the cell. Its main component, peptidoglycan (PG), is essential for cell division and wall elongation. Penicillin-binding proteins (PBPs) catalyze the last steps of PG biosynthesis, namely the polymerization of glycan chains and the cross-linking of stem peptides, and can be either monofunctional or bifunctional. Their action is coordinated with that of other enzymes essential for cell-wall biosynthesis, such as lytic transglycosylases (LT). Here, we have studied SltB1, an LT from Pseudomonas aeruginosa, and identified that it forms a complex with PBP2, a monofunctional enzyme, which requires the presence of Ca2+. In addition, we have solved the structure of SltB1 to a high resolution, and identified that it harbors an EF-hand like motif containing a Ca2+ ion displaying bipyramidal coordination. These studies provide initial structural details that shed light on the interactions between the PG biosynthesis enzymes in P. aeruginosa.
Original languageEnglish
Pages (from-to)298-305
Number of pages8
JournalMicrobial Drug Resistance
Volume18
Issue number3
DOIs
Publication statusPublished - 5 Jun 2012
Externally publishedYes

Cite this

Nikolaidis, Ioulia ; Izore, Thierry ; Job, Viviana ; Thielens, Nicole ; Breukink, Eefjan ; Dessen, Andrea. / Calcium-dependent complex formation between PBP2 and Lytic transglycosylase SltB1 of Pseudomonas aeruginosa. In: Microbial Drug Resistance. 2012 ; Vol. 18, No. 3. pp. 298-305.
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Calcium-dependent complex formation between PBP2 and Lytic transglycosylase SltB1 of Pseudomonas aeruginosa. / Nikolaidis, Ioulia; Izore, Thierry; Job, Viviana; Thielens, Nicole; Breukink, Eefjan; Dessen, Andrea.

In: Microbial Drug Resistance, Vol. 18, No. 3, 05.06.2012, p. 298-305.

Research output: Contribution to journalArticleResearchpeer-review

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