Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype

Shu Wen Wen, Luize G. Lima, Richard J. Lobb, Emma L. Norris, Marcus L. Hastie, Sophie Krumeich, Andreas Möller

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect the cell-of-origin. However, for the exosomal protein content, there is limited evidence in the literature to verify this statement. Here, proteomic assessment combined with pathway-enrichment analysis is used to demonstrate that the protein cargo of exosomes reflects the epithelial/mesenchymal phenotype of secreting breast cancer cells. Given that epithelial-mesenchymal plasticity is known to implicate various stages of cancer progression, the results suggest that breast cancer subtypes with distinct epithelial and mesenchymal phenotypes may be distinguished by directly assessing the protein content of exosomes. Additionally, the work is a substantial step toward verifying the statement that cell-derived exosomes reflect the phenotype of the cells-of-origin.

Original languageEnglish
Article number1800180
Number of pages9
JournalProteomics
Volume19
Issue number8
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • breast cancer
  • epithelial-to-mesenchymal transition
  • exosomes
  • proteomic

Cite this

Wen, S. W., Lima, L. G., Lobb, R. J., Norris, E. L., Hastie, M. L., Krumeich, S., & Möller, A. (2019). Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype. Proteomics, 19(8), [1800180]. https://doi.org/10.1002/pmic.201800180
Wen, Shu Wen ; Lima, Luize G. ; Lobb, Richard J. ; Norris, Emma L. ; Hastie, Marcus L. ; Krumeich, Sophie ; Möller, Andreas. / Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype. In: Proteomics. 2019 ; Vol. 19, No. 8.
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abstract = "A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect the cell-of-origin. However, for the exosomal protein content, there is limited evidence in the literature to verify this statement. Here, proteomic assessment combined with pathway-enrichment analysis is used to demonstrate that the protein cargo of exosomes reflects the epithelial/mesenchymal phenotype of secreting breast cancer cells. Given that epithelial-mesenchymal plasticity is known to implicate various stages of cancer progression, the results suggest that breast cancer subtypes with distinct epithelial and mesenchymal phenotypes may be distinguished by directly assessing the protein content of exosomes. Additionally, the work is a substantial step toward verifying the statement that cell-derived exosomes reflect the phenotype of the cells-of-origin.",
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Wen, SW, Lima, LG, Lobb, RJ, Norris, EL, Hastie, ML, Krumeich, S & Möller, A 2019, 'Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype' Proteomics, vol. 19, no. 8, 1800180. https://doi.org/10.1002/pmic.201800180

Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype. / Wen, Shu Wen; Lima, Luize G.; Lobb, Richard J.; Norris, Emma L.; Hastie, Marcus L.; Krumeich, Sophie; Möller, Andreas.

In: Proteomics, Vol. 19, No. 8, 1800180, 01.04.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Krumeich, Sophie

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Wen SW, Lima LG, Lobb RJ, Norris EL, Hastie ML, Krumeich S et al. Breast Cancer-Derived Exosomes Reflect the Cell-of-Origin Phenotype. Proteomics. 2019 Apr 1;19(8). 1800180. https://doi.org/10.1002/pmic.201800180