Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults

Robyn N Larsen, Bronwyn A Kingwell, Carol Vivien Robinson, Louise Hammond, Ester Cerin, Jonathan E Shaw, Genevieve N Healy, Marc T Hamilton, Neville Owen, David W Dunstan

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Abstract

To compare the cumulative (3-day) effect of prolonged sitting on metabolic responses during a mixed meal tolerance test (MTT), with sitting that is regularly interrupted with brief bouts of light-intensity walking. Overweight/obese adults (n=19) were recruited for a randomized, 3-day, outpatient, cross-over trial involving: (1) 7-h days of uninterrupted sitting (SIT); and (2) 7-h days of sitting with light-intensity activity breaks [BREAKS; 2-min of treadmill walking (3.2 km/h) every 20 min (total: 17 breaks/day)]. On days 1 and 3, participants underwent a MTT (75 g of carbohydrate, 50 g of fat) and the incremental area under the curve (iAUC) was calculated from hourly blood samples. Generalized estimating equation (GEE) models were adjusted for gender, body mass index (BMI), energy intake, treatment order and pre-prandial values to determine effects of time, condition and time x condition. The glucose iAUC was 1.3 +/- 0.5 and 1.5 +/- 0.5 mmol.h.l(-1) (mean differences +/- S.E.M.) higher in SIT compared with BREAKS on days 1 and 3 respectively (condition effect: P=0.001), with no effect of time (P=0.48) or time x condition (P=0.8). The insulin iAUC was also higher on both days in SIT (day 1: 151 +/- 73, day 3: 91 +/- 73 pmol.h.l(-1), P=0.01), with no effect of time (P=0.52) or time x condition (P=0.71). There was no between-treatment difference in triglycerides (triacylglycerols) iAUC. There were significant between-condition effects but no temporal change in metabolic responses to MTT, indicating that breaking up of sitting over 3 days sustains, but does not enhance, the lowering of postprandial glucose and insulin.
Original languageEnglish
Pages (from-to)117 - 127
Number of pages11
JournalClinical Science
Volume129
Issue number2
DOIs
Publication statusPublished - 2015

Cite this

@article{39cd873f50cc452d98702b15871c42a4,
title = "Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults",
abstract = "To compare the cumulative (3-day) effect of prolonged sitting on metabolic responses during a mixed meal tolerance test (MTT), with sitting that is regularly interrupted with brief bouts of light-intensity walking. Overweight/obese adults (n=19) were recruited for a randomized, 3-day, outpatient, cross-over trial involving: (1) 7-h days of uninterrupted sitting (SIT); and (2) 7-h days of sitting with light-intensity activity breaks [BREAKS; 2-min of treadmill walking (3.2 km/h) every 20 min (total: 17 breaks/day)]. On days 1 and 3, participants underwent a MTT (75 g of carbohydrate, 50 g of fat) and the incremental area under the curve (iAUC) was calculated from hourly blood samples. Generalized estimating equation (GEE) models were adjusted for gender, body mass index (BMI), energy intake, treatment order and pre-prandial values to determine effects of time, condition and time x condition. The glucose iAUC was 1.3 +/- 0.5 and 1.5 +/- 0.5 mmol.h.l(-1) (mean differences +/- S.E.M.) higher in SIT compared with BREAKS on days 1 and 3 respectively (condition effect: P=0.001), with no effect of time (P=0.48) or time x condition (P=0.8). The insulin iAUC was also higher on both days in SIT (day 1: 151 +/- 73, day 3: 91 +/- 73 pmol.h.l(-1), P=0.01), with no effect of time (P=0.52) or time x condition (P=0.71). There was no between-treatment difference in triglycerides (triacylglycerols) iAUC. There were significant between-condition effects but no temporal change in metabolic responses to MTT, indicating that breaking up of sitting over 3 days sustains, but does not enhance, the lowering of postprandial glucose and insulin.",
author = "Larsen, {Robyn N} and Kingwell, {Bronwyn A} and Robinson, {Carol Vivien} and Louise Hammond and Ester Cerin and Shaw, {Jonathan E} and Healy, {Genevieve N} and Hamilton, {Marc T} and Neville Owen and Dunstan, {David W}",
year = "2015",
doi = "10.1042/CS20140790",
language = "English",
volume = "129",
pages = "117 -- 127",
journal = "Clinical Science and Molecular Medicine",
issn = "0009-9287",
publisher = "Portland Press",
number = "2",

}

Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults. / Larsen, Robyn N; Kingwell, Bronwyn A; Robinson, Carol Vivien; Hammond, Louise; Cerin, Ester; Shaw, Jonathan E; Healy, Genevieve N; Hamilton, Marc T; Owen, Neville; Dunstan, David W.

In: Clinical Science, Vol. 129, No. 2, 2015, p. 117 - 127.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Breaking up of prolonged sitting over three days sustains, but does not enhance, lowering of postprandial plasma glucose and insulin in overweight and obese adults

AU - Larsen, Robyn N

AU - Kingwell, Bronwyn A

AU - Robinson, Carol Vivien

AU - Hammond, Louise

AU - Cerin, Ester

AU - Shaw, Jonathan E

AU - Healy, Genevieve N

AU - Hamilton, Marc T

AU - Owen, Neville

AU - Dunstan, David W

PY - 2015

Y1 - 2015

N2 - To compare the cumulative (3-day) effect of prolonged sitting on metabolic responses during a mixed meal tolerance test (MTT), with sitting that is regularly interrupted with brief bouts of light-intensity walking. Overweight/obese adults (n=19) were recruited for a randomized, 3-day, outpatient, cross-over trial involving: (1) 7-h days of uninterrupted sitting (SIT); and (2) 7-h days of sitting with light-intensity activity breaks [BREAKS; 2-min of treadmill walking (3.2 km/h) every 20 min (total: 17 breaks/day)]. On days 1 and 3, participants underwent a MTT (75 g of carbohydrate, 50 g of fat) and the incremental area under the curve (iAUC) was calculated from hourly blood samples. Generalized estimating equation (GEE) models were adjusted for gender, body mass index (BMI), energy intake, treatment order and pre-prandial values to determine effects of time, condition and time x condition. The glucose iAUC was 1.3 +/- 0.5 and 1.5 +/- 0.5 mmol.h.l(-1) (mean differences +/- S.E.M.) higher in SIT compared with BREAKS on days 1 and 3 respectively (condition effect: P=0.001), with no effect of time (P=0.48) or time x condition (P=0.8). The insulin iAUC was also higher on both days in SIT (day 1: 151 +/- 73, day 3: 91 +/- 73 pmol.h.l(-1), P=0.01), with no effect of time (P=0.52) or time x condition (P=0.71). There was no between-treatment difference in triglycerides (triacylglycerols) iAUC. There were significant between-condition effects but no temporal change in metabolic responses to MTT, indicating that breaking up of sitting over 3 days sustains, but does not enhance, the lowering of postprandial glucose and insulin.

AB - To compare the cumulative (3-day) effect of prolonged sitting on metabolic responses during a mixed meal tolerance test (MTT), with sitting that is regularly interrupted with brief bouts of light-intensity walking. Overweight/obese adults (n=19) were recruited for a randomized, 3-day, outpatient, cross-over trial involving: (1) 7-h days of uninterrupted sitting (SIT); and (2) 7-h days of sitting with light-intensity activity breaks [BREAKS; 2-min of treadmill walking (3.2 km/h) every 20 min (total: 17 breaks/day)]. On days 1 and 3, participants underwent a MTT (75 g of carbohydrate, 50 g of fat) and the incremental area under the curve (iAUC) was calculated from hourly blood samples. Generalized estimating equation (GEE) models were adjusted for gender, body mass index (BMI), energy intake, treatment order and pre-prandial values to determine effects of time, condition and time x condition. The glucose iAUC was 1.3 +/- 0.5 and 1.5 +/- 0.5 mmol.h.l(-1) (mean differences +/- S.E.M.) higher in SIT compared with BREAKS on days 1 and 3 respectively (condition effect: P=0.001), with no effect of time (P=0.48) or time x condition (P=0.8). The insulin iAUC was also higher on both days in SIT (day 1: 151 +/- 73, day 3: 91 +/- 73 pmol.h.l(-1), P=0.01), with no effect of time (P=0.52) or time x condition (P=0.71). There was no between-treatment difference in triglycerides (triacylglycerols) iAUC. There were significant between-condition effects but no temporal change in metabolic responses to MTT, indicating that breaking up of sitting over 3 days sustains, but does not enhance, the lowering of postprandial glucose and insulin.

UR - http://www.clinsci.org/content/129/2/117.full-text.pdf

U2 - 10.1042/CS20140790

DO - 10.1042/CS20140790

M3 - Article

VL - 129

SP - 117

EP - 127

JO - Clinical Science and Molecular Medicine

JF - Clinical Science and Molecular Medicine

SN - 0009-9287

IS - 2

ER -