Abstract
A novel midchain-functional chain transfer agent was synthesized and used as a reversible addition-fragmentation chain transfer polymerization agent to prepare branched poly(N-(2-hydroxypropyl) methacrylamide) (PHPMA). The P(HPMA) contained a thio-reactive group, namely, pyridyldisulfide midchain. The synthesized polymers were fully analyzed by 1H NMR, GPC, and hydrolysis, confirming well-defined structures (predesigned molecular weights, narrow polydispersities, and high functionalization efficiencies). These midfunctionalized polymers were incubated with bovine serum albumin to generate protein-polymer conjugates. This straightforward methodology for the synthesis of branched midfunctionalized polymers for protein conjugation has the potential to enhance the properties of polymer-protein conjugates by improving biomolecule stability and enhancing circulation time.
Original language | English |
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Pages (from-to) | 2847 - 2851 |
Number of pages | 5 |
Journal | Biomacromolecules |
Volume | 10 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |