Branched polymer-protein conjugates made from mid-chain-functional P(HPMA)

Lei Tao, Jingquan Liu, Thomas Paul Davis

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90 Citations (Scopus)


A novel midchain-functional chain transfer agent was synthesized and used as a reversible addition-fragmentation chain transfer polymerization agent to prepare branched poly(N-(2-hydroxypropyl) methacrylamide) (PHPMA). The P(HPMA) contained a thio-reactive group, namely, pyridyldisulfide midchain. The synthesized polymers were fully analyzed by 1H NMR, GPC, and hydrolysis, confirming well-defined structures (predesigned molecular weights, narrow polydispersities, and high functionalization efficiencies). These midfunctionalized polymers were incubated with bovine serum albumin to generate protein-polymer conjugates. This straightforward methodology for the synthesis of branched midfunctionalized polymers for protein conjugation has the potential to enhance the properties of polymer-protein conjugates by improving biomolecule stability and enhancing circulation time.
Original languageEnglish
Pages (from-to)2847 - 2851
Number of pages5
Issue number10
Publication statusPublished - 2009
Externally publishedYes

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