Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains

Hyun Ah Kim, Stephanie Whittle, Seyoung S Lee, Hannah Chu, Shenpeng Zhang, Zihui Wei, Thiruma Arumugam, Antony Vinh, Grant R Drummond, Christopher G Sobey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.
Original languageEnglish
Number of pages12
JournalFrontiers in Cellular Neuroscience
Volume8
Issue number365
DOIs
Publication statusPublished - 2014

Cite this

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title = "Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains",
abstract = "Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.",
author = "Kim, {Hyun Ah} and Stephanie Whittle and Lee, {Seyoung S} and Hannah Chu and Shenpeng Zhang and Zihui Wei and Thiruma Arumugam and Antony Vinh and Drummond, {Grant R} and Sobey, {Christopher G}",
year = "2014",
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language = "English",
volume = "8",
journal = "Frontiers in Cellular Neuroscience",
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Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains. / Kim, Hyun Ah; Whittle, Stephanie; Lee, Seyoung S; Chu, Hannah; Zhang, Shenpeng; Wei, Zihui; Arumugam, Thiruma; Vinh, Antony; Drummond, Grant R; Sobey, Christopher G.

In: Frontiers in Cellular Neuroscience, Vol. 8, No. 365, 2014.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains

AU - Kim, Hyun Ah

AU - Whittle, Stephanie

AU - Lee, Seyoung S

AU - Chu, Hannah

AU - Zhang, Shenpeng

AU - Wei, Zihui

AU - Arumugam, Thiruma

AU - Vinh, Antony

AU - Drummond, Grant R

AU - Sobey, Christopher G

PY - 2014

Y1 - 2014

N2 - Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.

AB - Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.

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